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Protective effects of in vivo-expressed autotransporters against Bordetella pertussis infection.
- Source :
-
Microbiology and immunology [Microbiol Immunol] 2017 Sep; Vol. 61 (9), pp. 371-379. - Publication Year :
- 2017
-
Abstract
- Bordetella pertussis causes whooping cough, a severe and prolonged respiratory disease that results inhas high morbidity and mortality rates, particularly in developing countries. The number incidence of whooping cough cases is increasing in many countries despite high vaccine coverage. Causes for the re-emergence of the disease include the limited duration of protection conferred by the acellular pertussis vaccines (aP)s and pathogenic adaptations that involve antigenic divergence from vaccine strains. Therefore, current vaccines therefore need to be improved. In the present study, we focused on five autotransporters: namely SphB1, BatB, SphB2, Phg, and Vag8, which were previously found to be expressed by B. bronchiseptica during the course of infection in rats and examined their protective efficiencies as vaccine antigens. The passenger domains of these proteins were produced in recombinant forms and used as antigens. An intranasal murine challenge assay showed that immunization with a mixture of SphB1 and Vag8 (SV) significantly reduced bacterial load in the lower respiratory tract and a combination of aP and SV acts synergistically in effects of conferring protection against B. pertussis infection, implying that these antigens have potential as components to for improvinge th the currently available acellular pertussis vaccine.<br /> (© 2017 The Societies and John Wiley & Sons Australia, Ltd.)
- Subjects :
- Animals
Antibodies, Bacterial immunology
Antigenic Variation immunology
Bacterial Load immunology
Bacterial Proteins immunology
Female
Mice
Mice, Inbred BALB C
Respiratory System immunology
Respiratory System microbiology
Serine Endopeptidases immunology
Vaccination
Whooping Cough immunology
Whooping Cough microbiology
Antigens, Bacterial immunology
Bordetella pertussis immunology
Pertussis Vaccine immunology
Type V Secretion Systems immunology
Whooping Cough prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1348-0421
- Volume :
- 61
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Microbiology and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 28752940
- Full Text :
- https://doi.org/10.1111/1348-0421.12504