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[Effects of Neurotrophin Receptor-interacting MAGE Homolog on Apoptosis of Intestinal Epithelial Cells].
- Source :
-
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2017 Jul; Vol. 48 (4), pp. 556-560. - Publication Year :
- 2017
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Abstract
- Objective: To explore whether neurotrophin receptor-interacting MAGE homolog (NRAGE) is involved in the intestinal ischemia-reperfusion (I/R) and its effect on the apoptosis of intestinal epithelial cells and the expression of occludin protein.<br />Methods: The level of NRAGE protein after the rat small intestine I/R was detected by immunohistochemical (IHC) In vivo . The level of NRAGE protein and mRNA in IEC-6 cells after hypoxia and reoxygenation were tested by Western blot and RT-PCR respectively in vitro . The IEC-6 cells were divided into four groups, including NRAGE overexpression by lentivirus infection (Lv- NRAGE group), interference (sh- NRAGE group), lentivirus control (Lv-control group), and normal control group without lentivirus infection (NC group). The apoptosis of IEC-6 cells after infection was analyzed by flow cytometry. The level of the tight junction protein occludin was detected by Western blot.<br />Results: The expression of NRAGE were highly increased in intestinal mucosa epithelial cells after I/R ( P <0.01). The proteins and mRNA levels of NRAGE were increased after 6 h of hypoxia in IEC-6 cells in vitro . Compared with the Lv-control group, the early apoptosis rate was raised ( P <0.01) and the level of occludin was reduced ( P <0.01) in Lv- NRAGE group; while the early apoptosis rate was reduced ( P <0.01) and the level of occludin was raised in sh- NRAGE group( P <0.001).<br />Conclusion: NRAGE may be involved in intestinal I/R and promote the apoptosis and decrease occludin expression of intestinal epithelial cells.
Details
- Language :
- Chinese
- ISSN :
- 1672-173X
- Volume :
- 48
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
- Publication Type :
- Academic Journal
- Accession number :
- 28752974