Back to Search Start Over

Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition.

Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition.

Authors :
Alberti A
Poulain J
Engelen S
Labadie K
Romac S
Ferrera I
Albini G
Aury JM
Belser C
Bertrand A
Cruaud C
Da Silva C
Dossat C
Gavory F
Gas S
Guy J
Haquelle M
Jacoby E
Jaillon O
Lemainque A
Pelletier E
Samson G
Wessner M
Acinas SG
Royo-Llonch M
Cornejo-Castillo FM
Logares R
Fernández-Gómez B
Bowler C
Cochrane G
Amid C
Hoopen PT
De Vargas C
Grimsley N
Desgranges E
Kandels-Lewis S
Ogata H
Poulton N
Sieracki ME
Stepanauskas R
Sullivan MB
Brum JR
Duhaime MB
Poulos BT
Hurwitz BL
Pesant S
Karsenti E
Wincker P
Source :
Scientific data [Sci Data] 2017 Aug 01; Vol. 4, pp. 170093. Date of Electronic Publication: 2017 Aug 01.
Publication Year :
2017

Abstract

A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009-2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world's planktonic ecosystems.

Details

Language :
English
ISSN :
2052-4463
Volume :
4
Database :
MEDLINE
Journal :
Scientific data
Publication Type :
Academic Journal
Accession number :
28763055
Full Text :
https://doi.org/10.1038/sdata.2017.93