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Using temperature-responsive zwitterionic surfactant to enhance the enzymatic hydrolysis of lignocelluloses and recover cellulase by cooling.
- Source :
-
Bioresource technology [Bioresour Technol] 2017 Nov; Vol. 243, pp. 1141-1148. Date of Electronic Publication: 2017 Jul 13. - Publication Year :
- 2017
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Abstract
- Some zwitterionic surfactants exhibit upper critical solution temperature (UCST) in aqueous solutions. For the zwitterionic surfactant solution mixed with cellulase, when its temperature is below UCST, the cellulase can be recovered by coprecipitation with zwitterionic surfactant. In this work, 3-(Hexadecyldimethylammonio) propanesulfonate (SB3-16) was selected to enhance the enzymatic hydrolysis of lignocelluloses and recover the cellulase. After adding 2mmol/L of SB3-16, the enzymatic digestibility of eucalyptus pretreated by dilute acid (Eu-DA) and by sulfite (Eu-SPORL) increased from 27.9% and 35.1% to 72.6% and 89.7%, respectively. The results showed that SB3-16 could reduce the non-productive adsorption of cellulase on hydrophobic interface, while it did not significantly inhibit the activity of cellulase. For the solution contained 1wt% SB3-16 and 200mg protein/L CTec2 cellulase, 55.2% of protein could be recovered by cooling. The filter paper activity of the recovered cellulase was 1.93FPU/mg protein, which was 95.8% of its initial activity.<br /> (Copyright © 2017. Published by Elsevier Ltd.)
- Subjects :
- Hydrolysis
Surface-Active Agents
Cellulase
Lignin
Temperature
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 243
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 28764128
- Full Text :
- https://doi.org/10.1016/j.biortech.2017.07.058