Back to Search
Start Over
5-HT modulates the rat mesenteric vasopressor outflow by 5-HT 1D sympatholytic receptors.
- Source :
-
Clinical and experimental pharmacology & physiology [Clin Exp Pharmacol Physiol] 2017 Dec; Vol. 44 (12), pp. 1224-1231. Date of Electronic Publication: 2017 Sep 18. - Publication Year :
- 2017
-
Abstract
- 5-hydroxytryptamine (5-HT) modulates noradrenergic activity in different cardiovascular territories, but its effect on the mesenteric vasopressor outflow has not yet been clarified. This study investigated the in vivo serotonergic influence, characterizing 5-HT receptors implicated, in sympathetic innervation of mesenteric vasculature. Wistar rats were anaesthetised and prepared for the in situ autoperfused rat mesentery, monitoring systemic blood pressure (SBP), heart rate (HR) and mesenteric perfusion pressure (MPP). Electrical stimulation of mesenteric sympathetic nerves resulted in frequency-dependent increases in MPP (9 ± 1.6, 25.7 ± 3.9 and 60.2 ± 5 mmHg for 2, 4 and 8 Hz, respectively), without altering SBP or HR. 5-HT (1-25 μg/kg), 5-carboxamidotryptamine (5-HT <subscript>1/7</subscript> agonist; 25 μg/kg) or L-694,247 (5-HT <subscript>1D</subscript> agonist; 1-25 μg/kg) i.a. bolus inhibited vasopressor responses by mesenteric nerves electrical stimulation, unlike i.a. bolus of agonists 8-OH-DPAT (5-HT <subscript>1A</subscript> ), CGS-12066B (5-HT <subscript>1B</subscript> ), BRL 54443 (5-HT <subscript>1e/1F</subscript> ), α-methyl-5-HT (5-HT <subscript>2</subscript> ), 1-PBG (5-HT <subscript>3</subscript> ), cisapride (5-HT <subscript>4</subscript> ) or AS-19 (5-HT <subscript>7</subscript> ) (25 μg/kg each). Interestingly, i.a. L-694,247 (25 μg/kg) also reduced the exogenous norepinephrine-induced vasoconstrictions. Pretreatment with selective 5-HT <subscript>1D</subscript> receptor antagonist, LY310762 (1 mg/kg, i.v.), completely abolished L-694,247- and 5-HT-induced mesenteric sympathoinhibition. Furthermore, ELISA analysis confirmed 5-HT <subscript>1D</subscript> receptors expression in mesenteric artery. These findings suggest that serotonergic mechanisms-induced sympathoinhibition of mesenteric noradrenergic outflow is mediated by pre and/or postjunctional 5-HT <subscript>1D</subscript> receptors.<br /> (© 2017 John Wiley & Sons Australia, Ltd.)
- Subjects :
- Animals
Electric Stimulation
In Vitro Techniques
Male
Mesenteric Arteries innervation
Mesenteric Arteries metabolism
Mesentery blood supply
Mesentery metabolism
Oxadiazoles pharmacology
Rats, Wistar
Tryptamines pharmacology
Vasoconstrictor Agents pharmacology
Mesenteric Arteries drug effects
Mesentery drug effects
Receptor, Serotonin, 5-HT1D metabolism
Serotonin pharmacology
Splanchnic Circulation drug effects
Sympathetic Nervous System drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1440-1681
- Volume :
- 44
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Clinical and experimental pharmacology & physiology
- Publication Type :
- Academic Journal
- Accession number :
- 28771848
- Full Text :
- https://doi.org/10.1111/1440-1681.12841