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Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre.

Authors :
Chien WM
Liu Y
Dinca AA
Chin MT
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1642, pp. 99-107.
Publication Year :
2017

Abstract

The technique of Cre-mediated DNA recombination at loxP sites has been used widely in manipulation of the genome in cultured cells and in living organisms. Local delivery of Cre recombinase protein tagged with a cell-penetrating (or permeable) peptide (Cre-CPP) has the advantage of additional spatial and temporal control when compared to genetic delivery methods. In this chapter, we describe protocols for injection-based intramuscular delivery of Cre-CPP dissolved in hydrogel to skeletal muscle and by ultrasound-guided injection to cardiac muscle in mice.

Details

Language :
English
ISSN :
1940-6029
Volume :
1642
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
28815496
Full Text :
https://doi.org/10.1007/978-1-4939-7169-5_7