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The ULK complex initiates autophagosome formation at phosphatidylinositol synthase-enriched ER subdomains.
- Source :
-
Autophagy [Autophagy] 2017 Oct 03; Vol. 13 (10), pp. 1795-1796. Date of Electronic Publication: 2017 Aug 17. - Publication Year :
- 2017
-
Abstract
- In our recent paper, we biochemically analyzed autophagosome-related membranes at the initiation stage of macroautophagy/autophagy using atg knockout (KO) cells and demonstrated that the ULK complex is recruited to 2 distinct membranes: the ER membrane and ATG9A-positive autophagosome precursors. We have also identified phosphatidylinositol synthase (PIS)-enriched ER subdomains as the initiation site of autophagosome formation. Based on these findings, we propose that the ULK complex, the PIS-enriched ER subdomain, and ATG9A vesicles together initiate autophagosome formation.
- Subjects :
- Adaptor Proteins, Signal Transducing metabolism
Adaptor Proteins, Signal Transducing physiology
Animals
Autophagy-Related Protein-1 Homolog physiology
Autophagy-Related Proteins metabolism
Autophagy-Related Proteins physiology
Cells, Cultured
Endoplasmic Reticulum chemistry
Endoplasmic Reticulum enzymology
Gene Knockdown Techniques
Humans
Membrane Microdomains chemistry
Membrane Microdomains enzymology
Protein-Tyrosine Kinases metabolism
Protein-Tyrosine Kinases physiology
Vesicular Transport Proteins metabolism
Vesicular Transport Proteins physiology
Autophagosomes physiology
Autophagy-Related Protein-1 Homolog metabolism
CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase metabolism
Endoplasmic Reticulum metabolism
Membrane Microdomains metabolism
Multiprotein Complexes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1554-8635
- Volume :
- 13
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Autophagy
- Publication Type :
- Academic Journal
- Accession number :
- 28816597
- Full Text :
- https://doi.org/10.1080/15548627.2017.1358344