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A Validated Reverse-Phase HPLC Method for Quantitative Determination of Ganoderic Acids A and B in Cultivated Strains of Ganoderma spp. (Agaricomycetes) Indigenous to India.
- Source :
-
International journal of medicinal mushrooms [Int J Med Mushrooms] 2017; Vol. 19 (5), pp. 457-465. - Publication Year :
- 2017
-
Abstract
- Twenty isolates of Ganoderma spp. indigenous to India were used in this study. A reverse-phase high-performance liquid chromatography (HPLC) method was used to quantify 2 of the most bioactive triterpenes, ganoderic acid A (GAA) and ganoderic acid B (GAB), among the cultivated Ganoderma spp. The HPLC analysis was performed on an Agilent 1260 Infinity HPLC system with a Zorbax C18 column, using gradient elution of acetonitrile and 0.1% acetic acid. The detection wavelength was set at 254 nm, with a flow rate of 0.6 mL/min. The method was validated according to the guidelines of International Conference on Harmonisation and produced satisfactory results. The amount of GAA varied from 827.50 to 2010.36 µg/g, whereas GAB varied from 16.64 to 916.89 µg/g. The developed method is simple, specific, accurate, and precise, and can be useful for qualitative and quantitative evaluation of ganoderic acids.
Details
- Language :
- English
- ISSN :
- 1940-4344
- Volume :
- 19
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of medicinal mushrooms
- Publication Type :
- Academic Journal
- Accession number :
- 28845774
- Full Text :
- https://doi.org/10.1615/IntJMedMushrooms.v19.i5.70