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The HBx gene of hepatitis B virus can influence hepatic microenvironment via exosomes by transferring its mRNA and protein.
- Source :
-
Virus research [Virus Res] 2017 Aug 15; Vol. 240, pp. 166-174. Date of Electronic Publication: 2017 Aug 25. - Publication Year :
- 2017
-
Abstract
- The cellular secretory vesicles known as 'exosomes' have emerged as key player in intercellular transport and communication between different eukaryotic in order to maintain body homeostasis. Many pathogenic viruses utilize exosome pathway to efficiently transfer bioactive components from infected cells to naïve cells. Here, we show that HBx can tweak the exosome biogenesis machinery both by enhancing neutral sphingomyelinase2 activity as well as by interacting with exosomal biomarkers such as neutral sphingomyelinase2, CD9 and CD81. The nano particle tracking analysis revealed enhanced secretion of exosomes by the HBx-expressing cells while confocal studies confirmed the co-localization of HBx with CD9 and CD63. Importantly, we observed the encapsulation of HBx mRNA and protein in these exosomes besides some other qualitative changes. The exosomal cargo secreted by HBx-expressing cells had a profound effect on the recipient hepatic cells including creation of a milieu conducive for cellular-transformation. Thus, the present study unfolds a novel role of HBx in intercellular communication by facilitating horizontal transfer of viral gene products and other host factors via exosomes in order to support viral spread and pathogenesis.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Exosomes genetics
Exosomes metabolism
Hepatitis B genetics
Hepatitis B metabolism
Hepatitis B virus genetics
Hepatitis B virus isolation & purification
Host-Pathogen Interactions
Humans
Liver metabolism
Metalloendopeptidases genetics
Metalloendopeptidases metabolism
Protein Binding
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Viral genetics
RNA, Viral metabolism
Tetraspanin 28 genetics
Tetraspanin 28 metabolism
Tetraspanin 29 genetics
Tetraspanin 29 metabolism
Trans-Activators genetics
Viral Proteins genetics
Viral Regulatory and Accessory Proteins
Exosomes virology
Hepatitis B virology
Hepatitis B virus metabolism
Liver virology
Trans-Activators metabolism
Viral Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7492
- Volume :
- 240
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 28847700
- Full Text :
- https://doi.org/10.1016/j.virusres.2017.08.009