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Identification of Taurine-Responsive Genes in Murine Liver Using the Cdo1-Null Mouse Model.

Authors :
Stipanuk MH
Jurkowska H
Niewiadomski J
Mazor KM
Roman HB
Hirschberger LL
Source :
Advances in experimental medicine and biology [Adv Exp Med Biol] 2017; Vol. 975 Pt 1, pp. 475-495.
Publication Year :
2017

Abstract

The cysteine dioxygenase (Cdo1)-null mouse is unable to synthesize hypotaurine and taurine by the cysteine/cysteine sulfinate pathway and has very low taurine levels in all tissues. The lack of taurine is associated with a lack of taurine conjugation of bile acids, a dramatic increase in the total and unconjugated hepatic bile acid pools, and an increase in betaine and other molecules that serve as organic osmolytes. We used the Cdo1-mouse model to determine the effects of taurine deficiency on expression of proteins involved in sulfur amino acid and bile acid metabolism. We identified cysteine sulfinic acid decarboxylase (Csad), betaine:homocysteine methytransferase (Bhmt), cholesterol 7α-hydroxylase (Cyp7a1), and cytochrome P450 3A11 (Cyp3a11) as genes whose hepatic expression is strongly regulated in response to taurine depletion in the Cdo1-null mouse. Dietary taurine supplementation of Cdo1-null mice restored hepatic levels of these four proteins and their respective mRNAs to wild-type levels, whereas dietary taurine supplementation had no effect on abundance of these proteins or mRNAs in wild-type mice.

Details

Language :
English
ISSN :
0065-2598
Volume :
975 Pt 1
Database :
MEDLINE
Journal :
Advances in experimental medicine and biology
Publication Type :
Academic Journal
Accession number :
28849476
Full Text :
https://doi.org/10.1007/978-94-024-1079-2_38