Back to Search
Start Over
Astragalus Granule Prevents Ca 2+ Current Remodeling in Heart Failure by the Downregulation of CaMKII.
- Source :
-
Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2017; Vol. 2017, pp. 7517358. Date of Electronic Publication: 2017 Aug 10. - Publication Year :
- 2017
-
Abstract
- Background: Astragalus was broadly used for treating heart failure (HF) and arrhythmias in East Asia for thousands of years. Astragalus granule (AG), extracted from Astragalus , shows beneficial effect on the treatment of HF in clinical research. We hypothesized that administration of AG prevents the remodeling of L-type Ca <superscript>2+</superscript> current ( I <subscript>Ca-L</subscript> ) in HF mice by the downregulation of Ca <superscript>2+</superscript> /calmodulin-dependent protein kinase II (CaMKII).<br />Methods: HF mice were induced by thoracic aortic constriction (TAC). After 4 weeks of AG treatment, cardiac function and QT interval were evaluated. Single cardiac ventricular myocyte was then isolated and whole-cell patch clamp was used to record action potential (AP) and I <subscript>Ca-L</subscript> . The expressions of L-type calcium channel alpha 1C subunit (Cav1.2), CaMKII, and phosphorylated protein kinase A (p-PKA) were examined by western blot.<br />Results: The failing heart manifested distinct electrical remodeling including prolonged repolarization time and altered I <subscript>Ca-L</subscript> kinetics. AG treatment attenuated this electrical remodeling, supported by AG-related shortened repolarization time, decreased peak I <subscript>Ca-L</subscript> , accelerated I <subscript>Ca-L</subscript> inactivation, and positive frequency-dependent I <subscript>Ca-L</subscript> facilitation. In addition, AG treatment suppressed the overexpression of CaMKII, but not p-PKA, in the failing heart.<br />Conclusion: AG treatment protected the failing heart against electrical remodeling and I <subscript>Ca-L</subscript> remodeling by downregulating CaMKII.
Details
- Language :
- English
- ISSN :
- 1741-427X
- Volume :
- 2017
- Database :
- MEDLINE
- Journal :
- Evidence-based complementary and alternative medicine : eCAM
- Publication Type :
- Academic Journal
- Accession number :
- 28855948
- Full Text :
- https://doi.org/10.1155/2017/7517358