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Integrin α8 and Pcdh15 act as a complex to regulate cilia biogenesis in sensory cells.
- Source :
-
Journal of cell science [J Cell Sci] 2017 Nov 01; Vol. 130 (21), pp. 3698-3712. Date of Electronic Publication: 2017 Sep 07. - Publication Year :
- 2017
-
Abstract
- The way an organism perceives its surroundings depends on sensory systems and the highly specialized cilia present in the neurosensory cells. Here, we describe the existence of an integrin α8 (Itga8) and protocadherin-15a (Pcdh15a) ciliary complex in neuromast hair cells in a zebrafish model. Depletion of the complex via downregulation or loss-of-function mutation leads to a dysregulation of cilia biogenesis and endocytosis. At the molecular level, removal of the complex blocks the access of Rab8a into the cilia as well as normal recruitment of ciliary cargo by centriolar satellites. These defects can be reversed by the introduction of a constitutively active form of Rhoa, suggesting that Itga8-Pcdh15a complex mediates its effect through the activation of this small GTPase and probably by the regulation of actin cytoskeleton dynamics. Our data points to a novel mechanism involved in the regulation of sensory cilia development, with the corresponding implications for normal sensory function.<br />Competing Interests: Competing interestsThe authors declare no competing or financial interests.<br /> (© 2017. Published by The Company of Biologists Ltd.)
- Subjects :
- Actin Cytoskeleton metabolism
Actin Cytoskeleton ultrastructure
Animals
Cadherin Related Proteins
Cadherins antagonists & inhibitors
Cadherins deficiency
Centrioles metabolism
Centrioles ultrastructure
Cilia ultrastructure
Disease Models, Animal
Endocytosis
Gene Expression Regulation, Developmental
Hair Cells, Auditory ultrastructure
Humans
Integrins antagonists & inhibitors
Integrins deficiency
Larva genetics
Larva growth & development
Larva metabolism
Monomeric GTP-Binding Proteins genetics
Monomeric GTP-Binding Proteins metabolism
Mutation
Protein Binding
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
Signal Transduction
Usher Syndromes genetics
Usher Syndromes metabolism
Usher Syndromes pathology
Zebrafish growth & development
Zebrafish metabolism
Zebrafish Proteins antagonists & inhibitors
Zebrafish Proteins deficiency
Zebrafish Proteins metabolism
rab GTP-Binding Proteins genetics
rab GTP-Binding Proteins metabolism
Cadherins genetics
Cilia metabolism
Hair Cells, Auditory metabolism
Integrins genetics
Zebrafish genetics
Zebrafish Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9137
- Volume :
- 130
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Journal of cell science
- Publication Type :
- Academic Journal
- Accession number :
- 28883094
- Full Text :
- https://doi.org/10.1242/jcs.206201