Start Over

Harmine promotes periodontal ligament cell-induced tissue regeneration.

Authors :: Lim HC
Cha BY
Song SU
Yun JH
Source :: Oral diseases [Oral Dis] 2018 Apr; Vol. 24 (3), pp. 456-464. Date of Electronic Publication: 2017 Oct 12.
Publication Year :: 2018
Abstract: Objective: to investigate whether harmine has a promotive effect on human periodontal ligament cells (hPDLCs)-induced tissue regeneration.<br />Materials and Methods: Various concentrations of harmine on hPDLCs proliferation were tested. Osteogenic and cementogenic characteristics were examined in hPDLC/rhBMP-2 and hPDLC/harmine by alizarin red S staining, real-time PCR, and Western blotting assay. The activity of harmine was investigated in an ectopic transplantation nude mouse model.<br />Results: We determined that 10 μM of harmine was the threshold concentration. hPDLC/harmine showed similar mineralized nodule formation in alizarin S staining compared to hPDLC/rhBMP-2. In real-time PCR, the highest gene expression level was observed for Runx2 in hPDLC/harmine at all time points. The level of CEMP-1 in hPDLC/harmine was higher at 7 days than hPDLCs alone. Thicker band of Runx2 in hPDLC/harmine was observed than in hPDLC/rhBMP-2 at 7 days by Western blotting. The band for CEMP-1 in hPDLC/harmine was thicker than hPDLCs alone at both 7 and 14 days. In ectopic transplantation, hPDLCs with harmine showed a comparable amount of mineralized tissue formation compared to rhBMP-2. hPDLCs with harmine or rhBMP-2 formed both bone and cementum-like tissue with Sharpey's fiber-like collagen insertion.<br />Conclusion: Harmine can be a potential candidate for promoting hPDLCs-induced tissue regeneration.<br /> (© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.)