Measurement of Kinetic Isotope Effects in an Enzyme-Catalyzed Reaction by Continuous-Flow Mass Spectrometry.

Authors :: Roberts KM
Fitzpatrick PF
Source :: Methods in enzymology [Methods Enzymol] 2017; Vol. 596, pp. 149-161. Date of Electronic Publication: 2017 Aug 04.
Publication Year :: 2017
Abstract: Kinetic isotope effects (KIEs) provide powerful probes of the mechanisms of enzyme-catalyzed reactions. In this chapter, we describe the use of continuous-flow mass spectrometry to determine the deuterium KIE for the enzyme N-acetylpolyamine oxidase based on the ratio of labeled and unlabeled products in mass spectra of whole reaction mixtures.<br /> (© 2017 Elsevier Inc. All rights reserved.)

Subjects :: Enzyme Assays instrumentation
Enzyme Assays methods
Kinetics
Mass Spectrometry instrumentation
Polyamine Oxidase
Biocatalysis
Deuterium chemistry
Mass Spectrometry methods
Oxidoreductases Acting on CH-NH Group Donors chemistry

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