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Identification of more than 500 RFLPs by screening random genomic clones.
- Source :
-
American journal of human genetics [Am J Hum Genet] 1988 Jan; Vol. 42 (1), pp. 143-59. - Publication Year :
- 1988
-
Abstract
- As part of our genome-mapping effort, we undertook a large-scale screening study to identify RFLPs useful as genetic markers. Some 1,664 single-copy or repeat-containing phage clones from a Charon 4A genomic library were tested for polymorphism against a panel of DNAs, from five unrelated individuals, digested with eight restriction enzymes. Approximately 30% (515) of the clones revealed polymorphism by Southern hybridization; 67 loci detected had PIC values greater than .5. Restriction enzymes MspI, TaqI, and RsaI were most efficient in detecting polymorphism within the 1-20-kb-fragment size range resolved. With only one exception each of the clones detected polymorphism originating from a single locus.
Details
- Language :
- English
- ISSN :
- 0002-9297
- Volume :
- 42
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- American journal of human genetics
- Publication Type :
- Academic Journal
- Accession number :
- 2892396