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Mild hypothermia provides Treg stability.

Authors :
Marek-Trzonkowska N
Piekarska K
Filipowicz N
Piotrowski A
Gucwa M
Vogt K
Sawitzki B
Siebert J
Trzonkowski P
Source :
Scientific reports [Sci Rep] 2017 Sep 20; Vol. 7 (1), pp. 11915. Date of Electronic Publication: 2017 Sep 20.
Publication Year :
2017

Abstract

Regulatory T cells (Tregs) play crucial role in maintenance of peripheral tolerance. Recent clinical trials confirmed safety and efficacy of Treg treatment of deleterious immune responses. However, Tregs lose their characteristic phenotype and suppressive potential during expansion ex vivo. Therefore, multiple research teams have been studding Treg biology in aim to improve their stability in vitro. In the current paper, we demonstrate that mild hypothermia of 33 °C induces robust proliferation of Tregs, preserves expression of FoxP3, CD25 and Helios, and prevents TSDR methylation during culture in vitro. Tregs expanded at 33 °C have stronger immunosuppressive potential and remarkably anti-inflammatory phenotype demonstrated by the whole transcriptome sequencing. These observations shed new light on impact of temperature on regulation of immune response. We show that just a simple change in temperature can preserve Treg stability, function and accelerate their proliferation, responding to unanswered question- how to preserve Treg stability in vitro.

Details

Language :
English
ISSN :
2045-2322
Volume :
7
Issue :
1
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
28931834
Full Text :
https://doi.org/10.1038/s41598-017-10151-1