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Mild hypothermia provides Treg stability.
- Source :
-
Scientific reports [Sci Rep] 2017 Sep 20; Vol. 7 (1), pp. 11915. Date of Electronic Publication: 2017 Sep 20. - Publication Year :
- 2017
-
Abstract
- Regulatory T cells (Tregs) play crucial role in maintenance of peripheral tolerance. Recent clinical trials confirmed safety and efficacy of Treg treatment of deleterious immune responses. However, Tregs lose their characteristic phenotype and suppressive potential during expansion ex vivo. Therefore, multiple research teams have been studding Treg biology in aim to improve their stability in vitro. In the current paper, we demonstrate that mild hypothermia of 33 °C induces robust proliferation of Tregs, preserves expression of FoxP3, CD25 and Helios, and prevents TSDR methylation during culture in vitro. Tregs expanded at 33 °C have stronger immunosuppressive potential and remarkably anti-inflammatory phenotype demonstrated by the whole transcriptome sequencing. These observations shed new light on impact of temperature on regulation of immune response. We show that just a simple change in temperature can preserve Treg stability, function and accelerate their proliferation, responding to unanswered question- how to preserve Treg stability in vitro.
- Subjects :
- Cell Proliferation radiation effects
Cells, Cultured
Cold Temperature
Forkhead Transcription Factors analysis
Gene Expression Profiling
Humans
Ikaros Transcription Factor analysis
Immune Tolerance
Interleukin-2 Receptor alpha Subunit analysis
T-Lymphocytes, Regulatory chemistry
Hypothermia
T-Lymphocytes, Regulatory physiology
T-Lymphocytes, Regulatory radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28931834
- Full Text :
- https://doi.org/10.1038/s41598-017-10151-1