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ZAAPS Program results for 2015: an activity and spectrum analysis of linezolid using clinical isolates from medical centres in 32 countries.
- Source :
-
The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2017 Nov 01; Vol. 72 (11), pp. 3093-3099. - Publication Year :
- 2017
-
Abstract
- Objectives: To report the linezolid in vitro activity obtained during the 2015 ZAAPS Program.<br />Methods: In total, 7587 organisms causing documented infections were consecutively collected in 65 centres in 32 ex-USA countries. Broth microdilution susceptibility testing was performed. Isolates displaying linezolid MIC results of ≥ 4 mg/L were molecularly characterized.<br />Results: Linezolid inhibited >99.9% of Staphylococcus aureus at ≤ 2 mg/L, with MIC50 results of 1 mg/L, regardless of methicillin resistance. A similar linezolid MIC50 result (0.5 mg/L) was observed for CoNS, with the vast majority of isolates (99.7%) also inhibited at ≤ 2 mg/L. Three CoNS (linezolid MIC, 16-64 mg/L) from Italy were found to contain alterations in the 23S rRNA and/or L3/L4 ribosomal proteins. One isolate also harboured cfr. Linezolid exhibited consistent modal MIC and MIC50 results (1 mg/L) for enterococci regardless of species or vancomycin resistance. One Enterococcus faecalis (linezolid MIC, 8 mg/L) from Galway, Ireland, carried optrA. One Enterococcus faecium (linezolid MIC, 16 mg/L) from Italy contained a G2576T mutation in the 23S rRNA. All Streptococcus pneumoniae, viridans group streptococci and β-haemolytic streptococci were inhibited by linezolid at ≤ 2, ≤ 2 and ≤ 1 mg/L, respectively, with equivalent MIC90 results (1 mg/L for all groups).<br />Conclusions: These results document the continued long-term and stable in vitro potency of linezolid and a limited number of isolates with decreased susceptibility to linezolid (MIC, ≥4 mg/L). The latter isolates showed primarily mutations in the 23S rRNA gene and/or L3/L4 proteins, with plasmid-mediated resistance (cfr and optrA) also present, albeit at a low prevalence.<br /> (© The Author 2017. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
- Subjects :
- Asia epidemiology
Canada epidemiology
Drug Resistance, Bacterial genetics
Enterococcus faecalis drug effects
Enterococcus faecalis genetics
Enterococcus faecalis isolation & purification
Europe epidemiology
Gram-Positive Bacteria genetics
Gram-Positive Bacterial Infections drug therapy
Gram-Positive Bacterial Infections epidemiology
Hospitals
Humans
Internationality
Intersectoral Collaboration
Microbial Sensitivity Tests
Plasmids
RNA, Ribosomal, 23S genetics
South America epidemiology
Staphylococcus aureus drug effects
Staphylococcus aureus genetics
Staphylococcus aureus isolation & purification
Streptococcus pneumoniae drug effects
Streptococcus pneumoniae genetics
Streptococcus pneumoniae isolation & purification
Anti-Bacterial Agents pharmacology
Epidemiological Monitoring
Gram-Positive Bacteria drug effects
Gram-Positive Bacteria isolation & purification
Linezolid pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2091
- Volume :
- 72
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of antimicrobial chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 28961701
- Full Text :
- https://doi.org/10.1093/jac/dkx251