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Adsorption of Insecticidal Crystal Protein Cry11Aa onto Nano-Mg(OH) 2 : Effects on Bioactivity and Anti-Ultraviolet Ability.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2017 Nov 01; Vol. 65 (43), pp. 9428-9434. Date of Electronic Publication: 2017 Oct 23. - Publication Year :
- 2017
-
Abstract
- The traditional Bacillus thuringiensis (Bt) formulations for field applications are not resistant to harsh environmental conditions. Hence, the active ingredients of the Bt bioinsecticides could degrade quickly and has low anti-ultraviolet ability in the field, which significantly limits its practical application. In the present study, we developed an efficient and stable delivery system for Bt Cry11Aa toxins. We coated Cry11Aa proteins with Mg(OH) <subscript>2</subscript> nanoparticles (MHNPs), and then assessed the effects of MHNPs on bioactivity and anti-ultraviolet ability of the Cry11Aa proteins. Our results indicated that MHNPs, like "coating clothes", could effectively protect the Cry protein and enhance the insecticidal bioactivity after UV radiation (the degradation rate was decreased from 64.29% to 16.67%). In addtion, MHNPs could improve the proteolysis of Cry11Aa in the midgut and aggravate the damage of the Cry protein to the gut epithelial cells, leading to increased insecticidal activity against Culex quinquefasciatus. Our results revealed that MHNPs, as an excellent nanocarrier, could substantially improve the insecticidal bioactivity and anti-ultraviolet ability of Cry11Aa.
- Subjects :
- Animals
Bacillus thuringiensis Toxins
Bacterial Proteins pharmacology
Culex drug effects
Drug Carriers chemistry
Drug Delivery Systems methods
Drug Stability
Endotoxins pharmacology
Hemolysin Proteins pharmacology
Insecticides pharmacology
Kinetics
Nanoparticles chemistry
Ultraviolet Rays
Bacterial Proteins chemistry
Drug Carriers radiation effects
Endotoxins chemistry
Hemolysin Proteins chemistry
Insecticides chemistry
Magnesium Hydroxide chemistry
Nanoparticles radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 65
- Issue :
- 43
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 29019656
- Full Text :
- https://doi.org/10.1021/acs.jafc.7b03410