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Polymerase Chain Reaction and Goldmann-Witmer Coefficient to Examine the Role of Epstein-Barr Virus in Uveitis.
- Source :
-
Ocular immunology and inflammation [Ocul Immunol Inflamm] 2019; Vol. 27 (1), pp. 108-113. Date of Electronic Publication: 2017 Oct 17. - Publication Year :
- 2019
-
Abstract
- Purpose: To use polymerase chain reaction (PCR) and Goldmann-Witmer Coefficient (GWC) calculation to search for evidence that Epstein-Barr virus (EBV) causes uveitis.<br />Methods: A prospective cross-sectional study where participants with positive multiplex EBV PCR results were further investigated by: 1) real-time PCR for EBV viral loads (VL) and 2) EBV GWC.<br />Results: Eleven of 106 consecutive uveitis patients (10.4%) had positive multiplex PCR for EBV on aqueous humor sampling and 7/11 (63.6%) were HIV-positive. Only 4/10 (40%) cases had detectable intraocular EBV VLs which were always lower than the blood or plasma VL. EBV GWC was negative in all 10 cases tested. In 9/11 (81.8%) of these cases an alternative, more plausible cause of uveitis was identified.<br />Conclusion: We found no evidence of active intraocular replication or antibody production to prove that EBV caused uveitis in these cases. In most cases an alternative treatable cause of uveitis was identified.
- Subjects :
- Adult
Antibodies, Viral blood
Aqueous Humor virology
Cross-Sectional Studies
DNA, Viral genetics
Epstein-Barr Virus Infections diagnosis
Eye Infections, Viral diagnosis
Female
HIV Infections diagnosis
HIV Infections virology
Herpesvirus 4, Human genetics
Herpesvirus 4, Human isolation & purification
Humans
Male
Middle Aged
Prospective Studies
Uveitis diagnosis
Viral Load
Enzyme-Linked Immunosorbent Assay methods
Epstein-Barr Virus Infections virology
Eye Infections, Viral virology
Herpesvirus 4, Human physiology
Polymerase Chain Reaction methods
Uveitis virology
Subjects
Details
- Language :
- English
- ISSN :
- 1744-5078
- Volume :
- 27
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Ocular immunology and inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 29039992
- Full Text :
- https://doi.org/10.1080/09273948.2017.1370653