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Bacillus kwashiorkori sp. nov., a new bacterial species isolated from a malnourished child using culturomics.
- Source :
-
MicrobiologyOpen [Microbiologyopen] 2018 Feb; Vol. 7 (1). Date of Electronic Publication: 2017 Oct 27. - Publication Year :
- 2018
-
Abstract
- Strain SIT6 <superscript>T</superscript> was isolated from the fecal flora of a severely malnourished child as part of a broad "culturomics" study aiming to maximize the culture conditions for the in-depth exploration of the human microbiota. An analysis of the 16S rRNA gene sequence showed that strain SIT6 <superscript>T</superscript> shared 94.1% 16S rRNA gene sequence similarity with Bacillus thermoamylovorans DKP <superscript>T</superscript> (NR&#95;029151), the phylogenetically closest type species. Colonies are creamy white, circular, 4-5 mm in diameter after cultivation at 37°C for 24 hr on 5% sheep blood-enriched Colombia agar. Growth occurs at temperatures in the range of 25-56°C (optimally at 37°C). Strain SIT6 <superscript>T</superscript> is a gram-positive, facultative anaerobic rod and motile by means of peritrichous flagella and sporulating; it is catalase and oxidase positive. The 2,784,637-bp-long genome, composed of 16 contigs, has a G+C content of 35.19%. Of the 2,646 predicted genes, 2,572 were protein-coding genes and 74 were RNAs. The major fatty acids are saturated species (15:0 iso, 16:0 and 17:0 anteiso). Of the 14 detected fatty acids, 11 are saturated, either linear or branched (iso and anteiso). Digital DNA-DNA hybridization (dDDH) estimation and average genomic identity of orthologous gene sequences (AGIOS) of the strain SIT6 <superscript>T</superscript> against genomes of the type strains of related species ranged between 18.6% and 38.3% and between 54.77% and 65.50%, respectively. According to our taxonogenomics results, we propose the creation of Bacillus kwashiorkori sp. nov. that contains the type strain SIT6 <superscript>T</superscript> (=CSUR P2452 <superscript>T</superscript> , =DSM 29059 <superscript>T</superscript> ).<br /> (© 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
- Subjects :
- Aerobiosis
Bacillus genetics
Bacillus physiology
Bacterial Typing Techniques
Base Composition
Cluster Analysis
Cytosol chemistry
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Fatty Acids analysis
Genome, Bacterial
Humans
Infant
Locomotion
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Temperature
Bacillus classification
Bacillus isolation & purification
Feces microbiology
Malnutrition
Subjects
Details
- Language :
- English
- ISSN :
- 2045-8827
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- MicrobiologyOpen
- Publication Type :
- Academic Journal
- Accession number :
- 29076642
- Full Text :
- https://doi.org/10.1002/mbo3.535