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Recombinant Lactobacillus plantarum expressing HA2 antigen elicits protective immunity against H9N2 avian influenza virus in chickens.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2017 Dec; Vol. 101 (23-24), pp. 8475-8484. Date of Electronic Publication: 2017 Oct 31. - Publication Year :
- 2017
-
Abstract
- Low pathogenic H9N2 subtype avian influenza virus (AIV) can lead to moderate respiratory symptoms and low egg production rates in poultry. Due to its immunologic suppression, other various infectious pathogens give rise to the co-infection of hosts, causing heavy economic losses in the commercial poultry industry in both China and worldwide. Therefore, it is time to explore a novel, safe, and effective vaccine. We have already made use of the surface of Lactobacillus plantarum to display AIV HA2 (NC8-pSIP409-pgsA'-HA2), which demonstrated that it has a good immunogenicity. In this study, by evaluating the immune protection effect of NC8-pSIP409-pgsA'-HA2 on chickens, we found that the hemagglutination inhibition (HI) antibodies, specificity IgG antibody in chickens, the sIgA titer in broncho alveolar lavage fluids (BALF), and the T cell response were increased notably after oral vaccination with NC8-pSIP409-pgsA'-HA2. In addition, weight loss, lung titers, and lung pathologies were improved when chickens were orally vaccinated with NC8-pSIP409-pgsA'-HA2 after challenge with H9N2 AIV. This strategy lays the foundation for the development of recombinant L. plantarum oral vaccines in the prevention of AIV.
- Subjects :
- Animals
Antibodies, Viral analysis
Antibodies, Viral blood
Antigens, Viral genetics
Antigens, Viral metabolism
Bronchoalveolar Lavage Fluid chemistry
Chickens
Drug Carriers
Genetic Vectors
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus genetics
Hemagglutinin Glycoproteins, Influenza Virus metabolism
Immunoglobulin A, Secretory analysis
Immunoglobulin G blood
Influenza A Virus, H9N2 Subtype genetics
Influenza Vaccines administration & dosage
Influenza Vaccines genetics
Influenza in Birds immunology
Influenza in Birds pathology
Influenza in Birds virology
Lactobacillus plantarum genetics
Lung pathology
Lung virology
Recombinant Proteins genetics
Recombinant Proteins metabolism
T-Lymphocytes immunology
Vaccines, Synthetic administration & dosage
Vaccines, Synthetic genetics
Vaccines, Synthetic immunology
Antigens, Viral immunology
Hemagglutinin Glycoproteins, Influenza Virus immunology
Influenza A Virus, H9N2 Subtype immunology
Influenza Vaccines immunology
Influenza in Birds prevention & control
Lactobacillus plantarum metabolism
Recombinant Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0614
- Volume :
- 101
- Issue :
- 23-24
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 29090342
- Full Text :
- https://doi.org/10.1007/s00253-017-8600-2