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Serum from CCl 4 -induced acute rat injury model induces differentiation of ADSCs towards hepatic cells and reduces liver fibrosis.
- Source :
-
Growth factors (Chur, Switzerland) [Growth Factors] 2017 Oct; Vol. 35 (4-5), pp. 144-160. Date of Electronic Publication: 2017 Nov 07. - Publication Year :
- 2017
-
Abstract
- Cellular therapies hold promise to alleviate liver diseases. This study explored the potential of allogenic serum isolated from rat with acute CCl <subscript>4</subscript> injury to differentiate adipose derived stem cells (ADSCs) towards hepatic lineage. Acute liver injury was induced by CCl <subscript>4</subscript> which caused significant increase in serum levels of VEGF, SDF1α and EGF. ADSCs were preconditioned with 3% serum isolated from normal and acute liver injury models. ADSCs showed enhanced expression of hepatic markers (AFP, albumin, CK8 and CK19). These differentiated ADSCs were transplanted intra-hepatically in CCl <subscript>4</subscript> -induced liver fibrosis model. After one month of transplantation, fibrosis and liver functions (alkaline phosphatase, ALAT and bilirubin) showed marked improvement in acute injury group. Elevated expression of hepatic (AFP, albumin, CK 18 and HNF4a) and pro survival markers (PCNA and VEGF) and improvement in liver architecture as deduced from results of alpha smooth muscle actin, Sirius red and Masson's trichome staining was observed.
- Subjects :
- Adipose Tissue cytology
Animals
Chemokine CCL4 toxicity
Hepatocytes cytology
Hepatocytes drug effects
Intercellular Signaling Peptides and Proteins metabolism
Liver Cirrhosis etiology
Mesenchymal Stem Cells drug effects
Rats
Rats, Sprague-Dawley
Cell Differentiation
Chemokine CCL4 blood
Culture Media, Conditioned pharmacology
Intercellular Signaling Peptides and Proteins pharmacology
Liver Cirrhosis therapy
Mesenchymal Stem Cell Transplantation methods
Mesenchymal Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1029-2292
- Volume :
- 35
- Issue :
- 4-5
- Database :
- MEDLINE
- Journal :
- Growth factors (Chur, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 29110545
- Full Text :
- https://doi.org/10.1080/08977194.2017.1392945