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Obesity-promoting and anti-thermogenic effects of neutrophil gelatinase-associated lipocalin in mice.
- Source :
-
Scientific reports [Sci Rep] 2017 Nov 14; Vol. 7 (1), pp. 15501. Date of Electronic Publication: 2017 Nov 14. - Publication Year :
- 2017
-
Abstract
- Neutrophil gelatinase-associated lipocalin (NGAL, lipocalin 2 or LCN2) is an iron carrier protein whose circulating level is increased by kidney injury, bacterial infection and obesity, but its metabolic consequence remains elusive. To study physiological role of LCN2 in energy homeostasis, we challenged female Lcn2 knockout (KO) and wild-type (WT) mice with high fat diet (HFD) or cold exposure. Under normal diet, physical constitutions of Lcn2 KO and WT mice were indistinguishable. During HFD treatment, Lcn2 KO mice exhibited larger brown adipose tissues (BAT), consumed more oxygen, ate more food and gained less body weights as compared to WT mice. When exposed to 4 °C, KO mice showed higher body temperature and more intense <superscript>18</superscript> F-fluorodeoxyglucose uptake in BAT, which were cancelled by β3 adrenergic receptor blocker or iron-loaded (but not iron-free) LCN2 administration. These findings suggest that circulating LCN2 possesses obesity-promoting and anti-thermogenic effects through inhibition of BAT activity in an iron-dependent manner.
- Subjects :
- Adipose Tissue, Brown drug effects
Adipose Tissue, Brown pathology
Adrenergic beta-3 Receptor Antagonists pharmacology
Animals
Biological Transport
Cold Temperature
Diet, High-Fat adverse effects
Eating genetics
Energy Metabolism genetics
Enterobactin pharmacology
Female
Fluorodeoxyglucose F18 metabolism
Gene Expression Regulation
Lipocalin-2 blood
Mice
Mice, Knockout
Obesity etiology
Obesity metabolism
Obesity pathology
Oxygen Consumption genetics
Propanolamines pharmacology
RNA, Messenger metabolism
Receptors, Adrenergic, beta-3 genetics
Receptors, Adrenergic, beta-3 metabolism
Signal Transduction
Adipose Tissue, Brown metabolism
Lipocalin-2 genetics
Obesity genetics
RNA, Messenger genetics
Thermogenesis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29138470
- Full Text :
- https://doi.org/10.1038/s41598-017-15825-4