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Statistically optimized ceftriaxone sodium biotransformation through Achromobacter xylosoxidans strain Cef6: an unusual insight for bioremediation.
- Source :
-
Journal of basic microbiology [J Basic Microbiol] 2018 Feb; Vol. 58 (2), pp. 120-130. Date of Electronic Publication: 2017 Nov 15. - Publication Year :
- 2018
-
Abstract
- The present study underlines a unique promising approach toward efficient biotransformation of ceftriaxone sodium (Ceftx), a highly frequent prescribed cephalosporin antibiotic, by a newly bacterium namely Achromobacter xylosoxidans strain Cef6 isolated from Ceftx contaminated raw materials in pharmaceutical industries. A three step sequential statistical-mathematical approach (Plackett-Burman design [PBD], Central Composite Design [CCD], and ridge-canonical analyses) was anticipated to optimize the biotransformation process. Ceftx concentration and medium volume: bottle volume ratio, two key determinants, significantly (p < 0.05) affected the process outcome deduced by regression analysis of PBD' data. CCD and ridge-canonical analyses localized the optimal levels of Ceftx concentration and medium volume: 250 ml bottle volume ratio to be 0.39 and 7.973 g Ceftx/L modified tryptic soy broth achieving Ceftx biotransformation (100%) after 39 h under aerobic static conditions at 30 °C, irrespectively deduced via HPLC analysis. Impressively, only one of five Ceftx byproducts was detected by the end of the biotransformation process. To the best of authors' knowledge, this is the first report addressing a detailed study regarding efficient biotransformation of Ceftx by single bacterium not bacterial consortium under aerobic conditions. Present data would greatly encourage applying this approach for decontamination of some Ceftx contaminated environmental sites.<br /> (© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Achromobacter denitrificans isolation & purification
Biodegradation, Environmental
Bioreactors microbiology
Biotransformation
Chromatography, High Pressure Liquid
Culture Media chemistry
Industrial Microbiology
Models, Statistical
Temperature
Time Factors
Achromobacter denitrificans metabolism
Anti-Bacterial Agents metabolism
Ceftriaxone metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4028
- Volume :
- 58
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of basic microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 29141102
- Full Text :
- https://doi.org/10.1002/jobm.201700497