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Transcriptome sequencing and differential gene expression analysis of the schistosome-transmitting snail Oncomelania hupensis inhabiting hilly and marshland regions.
- Source :
-
Scientific reports [Sci Rep] 2017 Nov 17; Vol. 7 (1), pp. 15809. Date of Electronic Publication: 2017 Nov 17. - Publication Year :
- 2017
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Abstract
- The freshwater snail Oncomelania hupensis is the unique intermediate host of the blood fluke Schistosoma japonicum, which is the major cause of schistosomiasis. The snail inhabits two contrasting environments: the hilly and marshland regions. The hilly snails are smaller in size and have the typical smooth shell, whereas the marshland snails are larger and possess the ribbed shell. To reveal the differences in gene expression between the hilly and marshland snails, a total of six snails, three per environment, were individually examined by RNA sequencing technology. All paired-end reads were assembled into contigs from which 34,760 unigenes were predicted. Based on single nucleotide polymorphisms, principal component analysis and neighbor-joining clustering revealed two distinct clusters of hilly and marshland snails. Analysis of expression changes between environments showed that upregulated genes relating to immunity and development were enriched in hilly snails, while those associated with reproduction were over-represented in marshland snails. Eight differentially expressed genes between the two types of snails were validated by qRT-PCR. Our study identified candidate genes that could be targets for future functional studies, and provided a link between expression profiling and ecological adaptation of the snail that may have implications for schistosomiasis control.
- Subjects :
- Animals
China
Cluster Analysis
Gene Expression Regulation
Gene Ontology
Geography
Molecular Sequence Annotation
Polymorphism, Single Nucleotide genetics
Reproducibility of Results
Ecosystem
Gene Expression Profiling
Schistosoma physiology
Sequence Analysis, RNA
Snails genetics
Snails parasitology
Transcriptome genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29150650
- Full Text :
- https://doi.org/10.1038/s41598-017-16084-z