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Leukotriene B4 indicates lung injury and on-going inflammatory changes after severe trauma in a porcine long-term model.
- Source :
-
Prostaglandins, leukotrienes, and essential fatty acids [Prostaglandins Leukot Essent Fatty Acids] 2017 Dec; Vol. 127, pp. 25-31. Date of Electronic Publication: 2017 Sep 22. - Publication Year :
- 2017
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Abstract
- Background: Recognizing patients at risk for pulmonary complications (PC) is of high clinical relevance. Migration of polymorphonuclear leukocytes (PMN) to inflammatory sites plays an important role in PC, and is tightly regulated by specific chemokines including interleukin (IL)-8 and other mediators such as leukotriene (LT)B4. Previously, we have reported that LTB4 indicated early patients at risk for PC after trauma. Here, the relevance of LTB4 to indicating lung integrity in a newly established long-term porcine severe trauma model (polytrauma, PT) was explored.<br />Methods: Twelve pigs (3 months old, 30 ± 5kg) underwent PT including standardized femur fracture, lung contusion, liver laceration, hemorrhagic shock, subsequent resuscitation and surgical fracture fixation. Six animals served as controls (sham). After 72h lung damage and inflammatory changes were assessed. LTB4 was determined in plasma before the experiment, immediately after trauma, and after 2, 4, 24 or 72h. Bronchoalveolar lavage (BAL)-fluid was collected prior and after the experiment.<br />Results: Lung injury, local gene expression of IL-8, IL-1β, IL-10, IL-18 and PMN-infiltration into lungs increased significantly in PT compared with sham. Systemic LTB4 increased markedly in both groups 4h after trauma. Compared with declined plasma LTB4 levels in sham, LTB4 increased further in PT after 72h. Similar increase was observed in BAL-fluid after PT.<br />Conclusions: In a severe trauma model, sustained changes in terms of lung injury and inflammation are determined at day 3 post-trauma. Specifically, increased LTB4 in this porcine long-term model indicated a rapid inflammatory alteration both locally and systemically. The results support the concept of LTB4 as a biomarker for PC after severe trauma and lung contusion.<br /> (Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- Animals
Bronchoalveolar Lavage Fluid
Disease Models, Animal
Humans
Inflammation etiology
Inflammation genetics
Inflammation physiopathology
Interleukins blood
Leukotriene B4 genetics
Lung metabolism
Lung physiopathology
Lung Injury etiology
Lung Injury genetics
Lung Injury physiopathology
Neutrophils metabolism
Swine
Wounds and Injuries complications
Wounds and Injuries physiopathology
Inflammation blood
Leukotriene B4 blood
Lung Injury blood
Wounds and Injuries blood
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2823
- Volume :
- 127
- Database :
- MEDLINE
- Journal :
- Prostaglandins, leukotrienes, and essential fatty acids
- Publication Type :
- Academic Journal
- Accession number :
- 29156155
- Full Text :
- https://doi.org/10.1016/j.plefa.2017.09.014