Back to Search
Start Over
Effect of exogenous progesterone on embryo size and ewe uterine gene expression in an ovine 'dam size' model of maternal constraint.
- Source :
-
Reproduction, fertility, and development [Reprod Fertil Dev] 2018 May; Vol. 30 (5), pp. 766-778. - Publication Year :
- 2018
-
Abstract
- Progesterone (P4), acting via its receptor, regulates uterine function and histotroph production, which are crucial to embryo growth. This study aimed to examine exogenous P4 effects on embryo size and differential endometrial gene expression at Day 19 of gestation using a 'dam size' sheep model of maternal constraint. Purebred Suffolk (S, genotypically large) embryos were transferred into recipient groups of Cheviot (C, genotypically small) or Suffolk ewes that had, or had not, been pre-treated with P4 from Days 0 to 6 of pregnancy. At Day 19S embryos were collected from four experimental groups: P4 pretreated S ewes (SP4; n=5), untreated S ewes (SnP4; n=15), P4 pretreated C ewes (CP4; n=7) and untreated C ewes (CnP4; n=21). Day-19 embryos from CP4 ewes were larger (P<0.05) than those from CnP4 ewes and similar in size (P>0.05) to embryos from SnP4 and SP4 ewes. Expression of mucin 1 (MUC1) and prostaglandin-endoperoxide synthase 2 (PTGS2) was upregulated in uterine horns ipsilateral to the corpus luteum from CP4 ewes. Prostaglandin receptor (PGR), MUC1 and PTGS2 expression was upregulated, whilst cathepsin L (CTSL) and radical S-adenosyl methionine domain-containing 2 (RSAD2) expression was downregulated in the ipsilateral horn of SP4 ewes. This suggests that pretreating ewes with exogenous P4 may alleviate early pregnancy maternal constraint via mechanisms that alter uterine function. However, further research is required to investigate the timing of P4 administration and its impact on conception rates.
- Subjects :
- Animals
Cyclooxygenase 2 genetics
Cyclooxygenase 2 metabolism
Embryo, Mammalian drug effects
Embryo, Mammalian metabolism
Female
Gene Expression Regulation drug effects
Mucin-1 genetics
Mucin-1 metabolism
Pregnancy
Receptors, Prostaglandin genetics
Receptors, Prostaglandin metabolism
Sheep
Uterus metabolism
Embryonic Development drug effects
Gene Expression drug effects
Progesterone pharmacology
Uterus drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1031-3613
- Volume :
- 30
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Reproduction, fertility, and development
- Publication Type :
- Academic Journal
- Accession number :
- 29157356
- Full Text :
- https://doi.org/10.1071/RD17096