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L-asparaginase isolated from Streptomyces ansochromogenes promotes Th1 profile and activates CD8 + T cells in human PBMC: an in vitro investigation.
- Source :
-
Journal of applied microbiology [J Appl Microbiol] 2018 May; Vol. 124 (5), pp. 1122-1130. Date of Electronic Publication: 2018 Feb 19. - Publication Year :
- 2018
-
Abstract
- Aims: A new L-asparaginase produced by Streptomyces ansochromogenes UFPEDA 3420 actinobacteria was used in this study against human lymphocyte cultures to evaluate the immunological profile induced by this enzyme.<br />Methods and Results: Cultures of lymphocytes were stimulated with S. ansochromogenes L-asparaginase, and cytotoxicity, cell viability, cell stimulation and cytokine production were analysed. This new S. ansochromogenes L-asparaginase induced activation and proliferation of the TCD8 <superscript>+</superscript> lymphocyte subset and produced higher TNF-α, IFN-γ, IL-2 and IL-10 levels in a 24-h assay.<br />Conclusion: Streptomyces ansochromogenes L-asparaginase is a promising molecule to be used in in vivo models and to deepen preclinical tests against acute lymphoblast leukaemia.<br />Significance and Impact of Study: L-asparaginase is an indispensable component of the chemotherapeutic treatment of acute lymphoblast leukaemia (ALL) and acute myeloid leukaemia (AML). Currently, drugs such as Asparaginase <superscript>®</superscript> , Kidrolase <superscript>®</superscript> , and Elspar <superscript>®</superscript> and Erwinase <superscript>®</superscript> are efficient against leukemic disease, but promote immunosuppression and other side effects in human organisms. Our purified S. ansochromogenes L-asparaginase showed promissory results inducing, in vitro, higher immunostimulation in human PBMC, especially in T CD8 <superscript>+</superscript> lymphocyte subsets.<br /> (© 2017 The Society for Applied Microbiology.)
- Subjects :
- Asparaginase isolation & purification
Asparaginase toxicity
CD8-Positive T-Lymphocytes immunology
Humans
Leukocytes, Mononuclear drug effects
Leukocytes, Mononuclear immunology
Asparaginase pharmacology
CD8-Positive T-Lymphocytes drug effects
Lymphocyte Activation drug effects
Streptomyces enzymology
Th1 Cells drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2672
- Volume :
- 124
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 29159986
- Full Text :
- https://doi.org/10.1111/jam.13644