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High-throughput and direct measurement of androgen levels using turbulent flow chromatography liquid chromatography-triple quadrupole mass spectrometry (TFC-LC-TQMS) to discover chemicals that modulate dihydrotestosterone production in human prostate cancer cells.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2018 Feb; Vol. 40 (2), pp. 263-270. Date of Electronic Publication: 2017 Nov 21. - Publication Year :
- 2018
-
Abstract
- Objectives: To develop a high-throughput screening system to measure the conversion of testosterone to dihydrotestosterone (DHT) in cultured human prostate cancer cells using turbulent flow chromatography liquid chromatography-triple quadrupole mass spectrometry (TFC-LC-TQMS).<br />Results: After optimizing the cell reaction system, this method demonstrated a screening capability of 103 samples, including 78 single compounds and 25 extracts, in less than 12 h without manual sample preparation. Consequently, fucoxanthin, phenethyl caffeate, and Curcuma longa L. extract were validated as bioactive chemicals that inhibited DHT production in cultured DU145 cells. In addition, naringenin boosted DHT production in DU145 cells.<br />Conclusion: The method can facilitate the discovery of bioactive chemicals that modulate the DHT production, and four phytochemicals are potential candidates of nutraceuticals to adjust DHT levels in male hormonal dysfunction.
- Subjects :
- Cell Line, Tumor
Cell Survival drug effects
Dihydrotestosterone metabolism
Drug Discovery
Flavanones chemistry
Flavanones pharmacology
High-Throughput Screening Assays methods
Humans
Male
Mass Spectrometry methods
Testosterone analysis
Testosterone metabolism
Xanthophylls chemistry
Xanthophylls pharmacology
Antineoplastic Agents chemistry
Antineoplastic Agents pharmacology
Chromatography, Liquid methods
Dihydrotestosterone analysis
Plant Extracts chemistry
Plant Extracts pharmacology
Prostatic Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 40
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 29164416
- Full Text :
- https://doi.org/10.1007/s10529-017-2480-5