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Cysteine-Rich Intestinal Protein 1 Silencing Inhibits Migration and Invasion in Human Colorectal Cancer.
- Source :
-
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2017; Vol. 44 (3), pp. 897-906. Date of Electronic Publication: 2017 Nov 24. - Publication Year :
- 2017
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Abstract
- Background/aims: Cysteine-rich intestinal protein 1 (CRIP1), a member of the LIM/double zinc finger protein family, is abnormally expressed in several tumour types. However, few data are available on the role of CRIP1 in cancer. In the present study, we aimed to investigate the expression profile and functions of CRIP1 in colorectal cancer.<br />Methods: To examine the protein expression level of CRIP1, immunohistochemistry (IHC) was performed on 56 pairs of colon cancer tissue samples. Western blotting was performed to investigate CRIP1 protein expression in four colon cancer cell lines. The endogenous expression of CRIP1 was suppressed using short interfering RNAs (siRNAs). Cell proliferation assays were used to determine whether CRIP1 silencing affected cell proliferation. Flow cytometry analysis was used to detect cell apoptosis. The effects of silencing CRIP1 on cell migration and invasion was detected using the transwell and wound-healing assays.<br />Results: IHC analysis showed that protein level of CRIP1 was significantly higher in tumour tissue samples than in paired non-tumour tissue samples and that the CRIP1 level was higher in metastatic tissue samples than in non-metastatic tissue samples. In addition, protein levels of CRIP1 were higher in highly metastatic colon cancer cell lines than in colon cancer cell lines with low metastasis. Further, CRIP1 silencing had no effect on cell proliferation or apoptosis in SW620 and HT29 cells. CRIP1 silencing suppressed cell migration and invasion obviously in SW620 and HT29 cells.<br />Conclusion: The present study provides new evidence that abnormal expression of CRIP1 might be related to the degree of metastasis in colorectal cancer and that CRIP1 silencing could effectively inhibit migration and invasion during colorectal cancer development. These findings might aid the development of a biomarker for colon cancer prognosis and metastasis, and thus help to treat this common type of cancer.<br /> (© 2017 The Author(s). Published by S. Karger AG, Basel.)
- Subjects :
- Aged
Carrier Proteins antagonists & inhibitors
Carrier Proteins genetics
Cell Line, Tumor
Cell Movement
Cell Proliferation
Colorectal Neoplasms metabolism
Female
HT29 Cells
Humans
Immunohistochemistry
LIM Domain Proteins antagonists & inhibitors
LIM Domain Proteins genetics
Male
Middle Aged
RNA, Small Interfering metabolism
Carrier Proteins metabolism
Colorectal Neoplasms pathology
LIM Domain Proteins metabolism
RNA Interference
Subjects
Details
- Language :
- English
- ISSN :
- 1421-9778
- Volume :
- 44
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 29179181
- Full Text :
- https://doi.org/10.1159/000485357