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Nanovaccine for immunotherapy and reduced hepatitis-B virus in humanized model.
- Source :
-
Artificial cells, nanomedicine, and biotechnology [Artif Cells Nanomed Biotechnol] 2018 Dec; Vol. 46 (8), pp. 2033-2042. Date of Electronic Publication: 2017 Nov 27. - Publication Year :
- 2018
-
Abstract
- Chronic Hepatitis B Virus (HBV) infections are severe with weak antiviral immune responses. The lack of an appropriate small animal model for chronic hepatitis, a major hurdle for studying the immunotolerance and immunopathogenesis induced by hepatitis B viral (HBV) infection. In this study, for enhancing the antibody production efficiency the prepared polymeric HBsAg-loaded nanoparticles (nanovaccine) will be tested in immune-deficit mice, which suffer from chronic Hepatitis B virus. Vaccination of Balb/c mice by this prepared nanoparticles that were engrafted with peripheral blood mononuclear cells (PBMCs), which was already lethally irradiated and transplanted by the bone marrow of NOD (knockout mice) mice. In the present study, after the vaccination detected the high frequencies of immunoglobulin G (IgG)-secreting B cells and mitogen-responsive interferon-Y (IFN-Y) secreting T cells in serum, determined by specific ELISA technique. During the entire observation period, unvaccinated animals showed lower concentration of specific IgG secreting B cells and IFN-Y secreting T cells found in comparison to vaccinated mice group. Chronic HBV carrier PBMCs transplanted into the chimera failed to produce antigen and increased the antibodies production due to vaccination. Furthermore, another advantage was that the viral gene expression and viral DNA replication was no longer observed in vaccinated group. This prepared nanovaccine formulations is better for the cure of Hepatitis B viral infection carrier. Therefore, specific memory responses were elicited by vaccination with Hepatitis B virus surface (HBsAg) antigen of chimeric mice transplanted with PBMCs derived from HBV donors.
- Subjects :
- Animals
Disease Models, Animal
Hepatitis B, Chronic metabolism
Hepatitis B, Chronic pathology
Humans
Leukocytes, Mononuclear transplantation
Mice
Mice, Inbred BALB C
Mice, Inbred NOD
Mice, Knockout
Hepatitis B Vaccines chemistry
Hepatitis B Vaccines pharmacology
Hepatitis B virus
Hepatitis B, Chronic therapy
Nanoparticles chemistry
Vaccination
Subjects
Details
- Language :
- English
- ISSN :
- 2169-141X
- Volume :
- 46
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Artificial cells, nanomedicine, and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 29179600
- Full Text :
- https://doi.org/10.1080/21691401.2017.1408118