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Nanoparticles in thermosensitive gel based composite nanosystem for ocular diseases.
- Source :
-
Drug delivery and translational research [Drug Deliv Transl Res] 2018 Apr; Vol. 8 (2), pp. 422-435. - Publication Year :
- 2018
-
Abstract
- The pentablock (PB) copolymers based composite nanosystems were designed to provide a long-term delivery of macromolecules to the back of the eye. A unique arrangement of each block (polyethylene glycol, polylactic acid, and polycaprolactone) with various molecular weights (PB-A and PB-B) was selected for the synthesis of nanoparticles (NPs) and thermosensitive gel (PB-C) by sequential ring-opening bulk copolymerization reaction. PB copolymers were characterized for their molecular weight and purity by <superscript>1</superscript> H-NMR spectroscopy and crystallinity by PXRD. The macromolecule model drugs [lysozyme (Lyz ~ 14.5 kDa), IgG-Fab (~ 50 kDa), and IgG (~ 150 kDa)] were selected to delineate the effect of molecular weights on in vitro release profile of nanoformulations. Lyz-, Fab-, and IgG-encapsulated NPs were prepared by double emulsion solvent evaporation method. The entrapment efficiency (EE%) and drug loading (DL%) of macromolecules was higher for PB-B copolymers due to its higher molecular weight and hydrophobicity compare to PB-A. The particle size range of NPs was ~ 200-270 nm. In vitro release profiles of Lyz-, Fab-, and IgG-encapsulated in NPs alone and NPs suspended in gel (composite nanosystem) demonstrated a minimal burst release and drug release over a long period. The effect of hydrodynamic diameter of macromolecules and hydrophobicity of PB copolymers was investigated on the release profile of nanosystems. In vitro biocompatibility study showed negligible cytokine (IL-1, IL-6, and TNF-α) release, which confirmed the safety of the PB copolymers. Based on the results, it is anticipated that long-term ocular delivery of macromolecules can be achieved through composite nanosystems.
- Subjects :
- Animals
Cytokines metabolism
Drug Compounding
Drug Liberation
Eye Diseases
Gels
Mice
RAW 264.7 Cells
Temperature
Immunoglobulin Fab Fragments administration & dosage
Immunoglobulin Fab Fragments chemistry
Immunoglobulin G administration & dosage
Immunoglobulin G chemistry
Muramidase administration & dosage
Muramidase chemistry
Nanoparticles administration & dosage
Nanoparticles chemistry
Polymers administration & dosage
Polymers chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2190-3948
- Volume :
- 8
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Drug delivery and translational research
- Publication Type :
- Academic Journal
- Accession number :
- 29181835
- Full Text :
- https://doi.org/10.1007/s13346-017-0435-y