Back to Search
Start Over
Th17 pathway in recent-onset autoimmune diabetes.
- Source :
-
Cellular immunology [Cell Immunol] 2018 Feb; Vol. 324, pp. 8-13. Date of Electronic Publication: 2017 Nov 15. - Publication Year :
- 2018
-
Abstract
- Aims: Evaluate the participation of IL-17 pathway in T1D pathogenesis. T helper 17 cells are potent, highly inflammatory cells that produce interleukin 17A (IL-17A), considered a mediator of various immune disorders. However, their role in Type 1 diabetes (T1D) pathogenesis in humans is not totally elucidated.<br />Methods: The expression of IL-17 Receptor A (IL-17RA) in peripheral T lymphocytes and IL-17A serum levels in recent-onset patients with T1D were compared with healthy controls. IL-17A gene variants were evaluated in a greater cohort.<br />Results: Patients with recent-onset T1D (less than 6 months of diagnosis) exhibited lower expression of IL-17RA in CD3+ T (% of cells = 31.3% × 43.6%; p = .041) and CD4+ T cells (11.1% × 25.2%; p = .0019) and lower number of IL-17RA in CD4+ T cells (MFI = 1.16 × 4.56; p = .03) than controls. IL-17RA expression in CD8+ T cells and IL-17A serum levels were similar in both groups. The coding regions and boundary intron sequences of IL17A were sequenced. Seventeen allelic variants, including three novel variants in exon 3 (3'UTR n) were identified, but no one was associated with T1D susceptibility, as well as the resulting haplotypes and diplotypes. The expression of IL-17RA was not correlated with metabolic variables (glucose and HbA1c levels) or pancreatic autoantibodies titers.<br />Conclusions: The lower expression of IL-17RA in CD3+ and CD4+ T cells suggests a reduced effect of IL-17A in immune response of recent-onset T1D patients, at least at peripheral tissues. IL-17A allelic variants were not related with T1D susceptibility.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Adolescent
Alleles
Brazil
CD4-Positive T-Lymphocytes metabolism
Child
Child, Preschool
Female
Gene Frequency genetics
Humans
Infant
Interleukin-17 analysis
Interleukin-17 blood
Male
Receptors, Interleukin-17 analysis
Receptors, Interleukin-17 blood
Receptors, Interleukin-17 genetics
Signal Transduction immunology
Signal Transduction physiology
Diabetes Mellitus, Type 1 metabolism
Interleukin-17 metabolism
Th17 Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2163
- Volume :
- 324
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 29183760
- Full Text :
- https://doi.org/10.1016/j.cellimm.2017.11.005