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Screening In Vitro Targets Related to Diabetes in Herbal Extracts from Peru: Identification of Active Compounds in Hypericum laricifolium Juss. by Offline High-Performance Liquid Chromatography.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2017 Nov 24; Vol. 18 (12). Date of Electronic Publication: 2017 Nov 24. - Publication Year :
- 2017
-
Abstract
- This study investigates in vitro targets related to diabetes in 30 herbal extracts from Peru, for the first time, using α-glucosidase, aldose reductase (AR) inhibitory assays and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging assays. Among the 30 herbal extracts, Hypericum laricifolium Juss. (HL) was the herb which showed more than 50% inhibition in all assays, presenting 97.2 ± 2.0%, 56.9 ± 5.6%, 81.9 ± 2.5%, and 58.8 ± 4.6% inhibition for the α-glucosidase, AR, DPPH, and ABTS assays, respectively. Finally, six bioactive compounds, namely, protocatechuic acid, chlorogenic acid, caffeic acid, kaempferol 3- O -glucuronide, quercetin, and kaempferol were identified in HL by offline high-performance liquid chromatography (HPLC). Quercetin exhibited the strongest inhibition in all enzyme assays and the strongest antioxidant activity. The results suggest that HL shows great potential for the complementary treatment of diabetes and its complications.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Aldehyde Reductase antagonists & inhibitors
Animals
Caffeic Acids analysis
Free Radical Scavengers pharmacology
Glycoside Hydrolase Inhibitors pharmacology
Hydroxybenzoates analysis
Hypoglycemic Agents pharmacology
Lens, Crystalline drug effects
Plant Extracts pharmacology
Rats
Rats, Sprague-Dawley
Free Radical Scavengers chemistry
Glycoside Hydrolase Inhibitors chemistry
Hypericum chemistry
Hypoglycemic Agents chemistry
Plant Extracts chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 18
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 29186785
- Full Text :
- https://doi.org/10.3390/ijms18122512