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Immunogenicity of recombinant vaccinia virus vaccines co-expressing GP3/GP5 of European PRRSV and Cap protein of PCV2 in pigs.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2018 Feb; Vol. 102 (3), pp. 1145-1154. Date of Electronic Publication: 2017 Nov 30. - Publication Year :
- 2018
-
Abstract
- Porcine reproductive and respiratory syndrome (PRRS) is almost always caused by the North American strain of PRRS virus (PRRSV) in China; the European genotype of PRRSV has emerged in China. The mixed infection of PRRSV and Porcine circovirus type 2 virus (PCV2) are always found in pigs and PRRSV-augmented PCV2 replication and serious clinical symptoms. Current vaccines cannot protect mixed European PRRSV and PCV2 infections. Therefore, the development of a safe and effective new vaccine to prevent and control the mixed infection of European PRRSV and PCV2 is both urgent and necessary. In this study, we developed a recombinant vaccinia vaccine co-expressing the GP3 and GP5 proteins of European PRRSV and the ORF2 protein of PCV2 and evaluated the immunogenicity and its protective effects and its inactivated vaccine in pigs. The recombinant vaccinia vaccine and its inactivated vaccine both elicited significant humoral and cellular immune responses with a higher level of specific antibody responses and T-lymphocyte proliferation than the control group. Furthermore, the pigs inoculated with the recombinant vaccinia vaccine were completely protected against challenge with 10 <superscript>5</superscript> TCID <subscript>50</subscript> of European PRRSV strain LV. These data suggest that the recombinant vaccinia vaccine is a potential candidate vaccine against European PRRSV and PCV2.
- Subjects :
- Animals
Antibodies, Neutralizing blood
Antibodies, Viral blood
China
Circoviridae Infections immunology
Circovirus genetics
Circovirus immunology
Europe
Genetic Vectors
Porcine Reproductive and Respiratory Syndrome immunology
Porcine respiratory and reproductive syndrome virus genetics
Recombinant Fusion Proteins administration & dosage
Recombinant Fusion Proteins immunology
Swine
Vaccinia virus genetics
Viral Envelope Proteins genetics
Circoviridae Infections prevention & control
Cyclic AMP Receptor Protein genetics
Immunogenicity, Vaccine
Porcine Reproductive and Respiratory Syndrome prevention & control
Porcine respiratory and reproductive syndrome virus immunology
Viral Envelope Proteins immunology
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0614
- Volume :
- 102
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 29189898
- Full Text :
- https://doi.org/10.1007/s00253-017-8661-2