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Relatedness of Antibodies to Peptides Containing Homocitrulline or Citrulline in Patients with Rheumatoid Arthritis.
- Source :
-
The Journal of rheumatology [J Rheumatol] 2018 Mar; Vol. 45 (3), pp. 302-309. Date of Electronic Publication: 2017 Dec 01. - Publication Year :
- 2018
-
Abstract
- Objective: Antibodies that target citrullinated protein/peptide (ACPA) and homocitrullinated/carbamylated protein/peptide (AHCPA) are associated with rheumatoid arthritis (RA). The relationship between ACPA and AHCPA remains unclear. We examined the expression and cross-reactivity of these antibodies using citrulline- and homocitrulline-containing synthetic peptides, CitJED and HomoCitJED, respectively, which have equal numbers of citrulline or homocitrulline residues on the same peptide backbone.<br />Methods: Serum from healthy subjects (n = 51) and patients with RA (n = 137), systemic lupus erythematosus (SLE; n = 37), and psoriatic arthritis (PsA; n = 37) were screened for IgG anti-CitJED and anti-HomoCitJED antibodies by ELISA. Cross-reactivity of these antibodies was examined by inhibition with various concentrations of CitJED and HomoCitJED.<br />Results: Out of 137 patients with RA, antibodies to CitJED and HomoCitJED were detected in 69 (50%) and 78 (57%), respectively. Anti-CitJED and HomoCitJED antibodies were 77% concordant and their levels were strongly correlated [Spearman correlation coefficient (r <subscript>s</subscript> ) = 0.6676]. Sera from 25/27 patients (93%) with RA were inhibited by both CitJED and HomoCitJED with equal or higher affinity for the cognate (homologous) peptide.<br />Conclusion: Antibodies to CitJED and HomoCitJED frequently occurred in RA, but were not found in SLE or PsA, suggesting that these antibodies are specific to RA. Cross-reactivity between anti-HomoCitJED and anti-CitJED antibodies suggests that ACPA and AHCPA are derived from the same B cell population and both may contribute to the pathogenesis of RA.
- Subjects :
- Adult
Aged
Aged, 80 and over
Analysis of Variance
Antibody Affinity immunology
Citrulline chemical synthesis
Citrulline immunology
Enzyme-Linked Immunosorbent Assay
Epitopes immunology
Female
Healthy Volunteers
Humans
Immunoglobulin G immunology
Male
Middle Aged
Peptides, Cyclic immunology
Statistics, Nonparametric
Young Adult
Arthritis, Psoriatic blood
Arthritis, Rheumatoid blood
Autoantibodies blood
Citrulline analogs & derivatives
Lupus Erythematosus, Systemic blood
Subjects
Details
- Language :
- English
- ISSN :
- 0315-162X
- Volume :
- 45
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of rheumatology
- Publication Type :
- Academic Journal
- Accession number :
- 29196382
- Full Text :
- https://doi.org/10.3899/jrheum.170446