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[Screening of adult Ph-like acute lymphoblastic leukemia by multiplex real-time quantitative PCR].
- Source :
-
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi [Zhonghua Xue Ye Xue Za Zhi] 2017 Nov 14; Vol. 38 (11), pp. 956-961. - Publication Year :
- 2017
-
Abstract
- Objective: To investigate the feasibility of multiplex real-time RT-PCR with fluorescent probes in early screening of Ph-like acute lymphoblastic leukemia (ALL) and analyze the clinical feature and prognos. Method: A total of 118 adult B-ALL patients diagnosed between October 2010 and March 2016 were enrolled in this study. Multiplex RT-PCR was used to detect the Ph-like ALL related fusion gene and CRLF2 expression in 58 BCR-ABL and MLL rearrangement negative patients. The clinical features, treatment response and prognosis were analyzed in Ph-like fusion gene positive and/or CRLF2 over-expression patients. Result: Among 58 patients, 9 patients (9/58, 15.5%) showed Ph-like ALL related fusion genes positive and 10 patients (10/58, 17.2%) showed CRLF2 over-expression. There were statistical differences in age, WBC count, immunophenotypes, cytogenetics and risk stratification among Ph-like fusion gene positive or CRLF2 over-expression patients, Ph(+) patients, MLL(+) patients and B-other patients. The 2-year overall survival rates were 65%, 47%, 64% and 74% respectively among these four groups ( P =0.043) . The 2-year relapse free survival rates were 51%, 39%, 62% and 70% respectively among these four groups ( P =0.010) . Conclusion: Routine screening of Ph-like ALL by multiplex RTPCR is feasible.
Details
- Language :
- Chinese
- ISSN :
- 0253-2727
- Volume :
- 38
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
- Publication Type :
- Academic Journal
- Accession number :
- 29224319
- Full Text :
- https://doi.org/10.3760/cma.j.issn.0253-2727.2017.11.011