Back to Search
Start Over
Effects of six substances on the growth and freeze-drying of Lactobacillus delbrueckii subsp. bulgaricus.
- Source :
-
Acta scientiarum polonorum. Technologia alimentaria [Acta Sci Pol Technol Aliment] 2017 Oct-Dec; Vol. 16 (4), pp. 403-412. - Publication Year :
- 2017
-
Abstract
- Background: The efficacy of Lactobacillus delbrueckii subsp. bulgaricus as starter cultures for the dairy industry depends largely on the number of viable and active cells. Freeze-drying is the most convenient and successful method to preserve the bacterial cells. However, not all strains survived during freeze-drying.<br />Methods: The effects of six substances including NaCl, sorbitol, mannitol, mannose, sodium glutamate, betaine added to the MRS medium on the growth and freeze-drying survival rate and viable counts of Lb. delbrueckii subsp. bulgaricus were studied through a single-factor test and Plackett-Burman design. Subsequently, the optimum freeze-drying conditions of Lb. delbrueckii subsp. bulgaricus were determined.<br />Results: Lb. delbrueckii subsp. bulgaricus survival rates were up to the maximum of 42.7%, 45.4%, 23.6%, while the concentrations of NaCl, sorbitol, sodium glutamate were 0.6%, 0.15%, 0.09%, respectively. In the optimum concentration, the viable counts in broth is 6.1, 6.9, 5.13 (&times;108 CFU/mL), respectively; the viable counts in freeze-drying power are 3.09, 5.2, 2.7 (&times;1010 CFU/g), respectively.<br />Conclusions: Three antifreeze factors including NaCl, sorbitol, sodium glutamate have a positive effect on the growth and freeze-drying of Lb. delbrueckii subsp. bulgaricus. The results are beneficial for developing Lb. delbrueckii subsp. bulgaricus.
- Subjects :
- Betaine pharmacology
Mannitol pharmacology
Microbial Viability
Sodium Chloride pharmacology
Sodium Glutamate pharmacology
Sorbitol pharmacology
Yogurt microbiology
Cryoprotective Agents pharmacology
Food Additives pharmacology
Freeze Drying
Lactobacillus delbrueckii drug effects
Lactobacillus delbrueckii growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1898-9594
- Volume :
- 16
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Acta scientiarum polonorum. Technologia alimentaria
- Publication Type :
- Academic Journal
- Accession number :
- 29241319
- Full Text :
- https://doi.org/10.17306/J.AFS.0512