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Effects of six substances on the growth and freeze-drying of Lactobacillus delbrueckii subsp. bulgaricus.

Authors :
Chen H
Huang J
Shi X
Li Y
Liu Y
Source :
Acta scientiarum polonorum. Technologia alimentaria [Acta Sci Pol Technol Aliment] 2017 Oct-Dec; Vol. 16 (4), pp. 403-412.
Publication Year :
2017

Abstract

Background: The efficacy of Lactobacillus delbrueckii subsp. bulgaricus as starter cultures for the dairy industry depends largely on the number of viable and active cells. Freeze-drying is the most convenient and successful method to preserve the bacterial cells. However, not all strains survived during freeze-drying.<br />Methods: The effects of six substances including NaCl, sorbitol, mannitol, mannose, sodium glutamate, betaine added to the MRS medium on the growth and freeze-drying survival rate and viable counts of Lb. delbrueckii subsp. bulgaricus were studied through a single-factor test and Plackett-Burman design. Subsequently, the optimum freeze-drying conditions of Lb. delbrueckii subsp. bulgaricus were determined.<br />Results: Lb. delbrueckii subsp. bulgaricus survival rates were up to the maximum of 42.7%, 45.4%, 23.6%, while the concentrations of NaCl, sorbitol, sodium glutamate were 0.6%, 0.15%, 0.09%, respectively. In the optimum concentration, the viable counts in broth is 6.1, 6.9, 5.13 (×108 CFU/mL), respectively; the viable counts in freeze-drying power are 3.09, 5.2, 2.7 (×1010 CFU/g), respectively.<br />Conclusions: Three antifreeze factors including NaCl, sorbitol, sodium glutamate have a positive effect on the growth and freeze-drying of Lb. delbrueckii subsp. bulgaricus. The results are beneficial for developing Lb. delbrueckii subsp. bulgaricus.

Details

Language :
English
ISSN :
1898-9594
Volume :
16
Issue :
4
Database :
MEDLINE
Journal :
Acta scientiarum polonorum. Technologia alimentaria
Publication Type :
Academic Journal
Accession number :
29241319
Full Text :
https://doi.org/10.17306/J.AFS.0512