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LncRNA GAS5-AS1 inhibits myofibroblasts activities in oral submucous fibrosis.

Authors :: Lin CY
Liao YW
Hsieh PL
Lu MY
Peng CY
Chu PM
Yang HW
Huang YF
Yu CC
Yu CH
Source :: Journal of the Formosan Medical Association = Taiwan yi zhi [J Formos Med Assoc] 2018 Aug; Vol. 117 (8), pp. 727-733. Date of Electronic Publication: 2017 Dec 15.
Publication Year :: 2018
Abstract: Background/purpose: Emerging research findings suggest that long non-coding RNAs (lncRNAs) are key regulators to fibrosis formation. Nevertheless, the role of lncRNA GAS5-AS1 in the progression of precancerous oral submucous fibrosis (OSF) remains to be elucidated.<br />Methods: Quantitative real-time PCR were used to examine the expression of GAS5-AS1 in OSF tissues. The activities of myofibroblasts, including collagen contractility and cell migration, as well as the marker α-smooth muscle actin (SMA) were assessed following overexpression of GAS5-AS1. Also, we analyzed the expression of Smad activity in order to gain insight into the downstream regulator.<br />Results: The level of GAS5-AS1 was found significantly downregulated in the OSF tissues and fibrotic buccal mucosal fibroblasts (fBMFs). Ectopic expression of GAS5-AS1 significantly reduced the abilities of collagen gel contraction and migration in fBMFs or arecoline-treated BMFs. Moreover, we have shown that overexpression of GAS5-AS1 inhibited the expression of p-Smad and the marker of myofibroblasts.<br />Conclusion: We showed the reduced expression of GAS5-AS1 in OSF tissues and demonstrated its effect on the myofibroblast activities and the level of p-Smad and α-SMA, indicating its potential contribution in OSF pathogenesis.<br /> (Copyright © 2017. Published by Elsevier B.V.)