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Evaluation of Biosynthesis, Accumulation and Antioxidant Activityof Vitamin E in Sweet Corn (Zea mays L.) during Kernel Development.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2017 Dec 20; Vol. 18 (12). Date of Electronic Publication: 2017 Dec 20. - Publication Year :
- 2017
-
Abstract
- Sweet corn kernels were used in this research to study the dynamics of vitamin E, by evaluatingthe expression levels of genes involved in vitamin E synthesis, the accumulation of vitamin E, and the antioxidant activity during the different stage of kernel development. Results showed that expression levels of <subscript>Zm</subscript> HPT and <subscript>Zm</subscript> TC genes increased, whereas <subscript>Zm</subscript> TMT gene dramatically decreased during kernel development. The contents of all the types of vitamin E in sweet corn had a significant upward increase during kernel development, and reached the highest level at 30 days after pollination (DAP). Amongst the eight isomers of vitamin E, the content of γ-tocotrienol was the highest, and increased by 14.9 folds, followed by α-tocopherolwith an increase of 22 folds, and thecontents of isomers γ-tocopherol, α-tocotrienol, δ-tocopherol,δ-tocotrienol, and β-tocopherol were also followed during kernel development. The antioxidant activity of sweet corn during kernel development was increased, and was up to 101.8 ± 22.3 μmol of α-tocopherol equivlent/100 g in fresh weight (FW) at 30 DAP. There was a positive correlation between vitamin E contents and antioxidant activity in sweet corn during the kernel development, and a negative correlation between the expressions of <subscript>Zm</subscript> TMT gene and vitamin E contents. These results revealed the relations amongst the content of vitamin E isomers and the gene expression, vitamin E accumulation, and antioxidant activity. The study can provide a harvesting strategy for vitamin E bio-fortification in sweet corn.<br />Competing Interests: The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; collection, analyses, or interpretation of data; writing of the manuscript and the decision to publish the results.
- Subjects :
- Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Plant Proteins genetics
Plant Proteins metabolism
Seeds chemistry
Seeds growth & development
Seeds metabolism
Vitamin E genetics
Vitamin E metabolism
Zea mays growth & development
Zea mays metabolism
Antioxidants analysis
Seeds genetics
Vitamin E biosynthesis
Zea mays genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 18
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 29261149
- Full Text :
- https://doi.org/10.3390/ijms18122780