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Cultured circulating tumor cells and their derived xenografts for personalized oncology.

Authors :
Wang R
Chu GCY
Mrdenovic S
Annamalai AA
Hendifar AE
Nissen NN
Tomlinson JS
Lewis M
Palanisamy N
Tseng HR
Posadas EM
Freeman MR
Pandol SJ
Zhau HE
Chung LWK
Source :
Asian journal of urology [Asian J Urol] 2016 Oct; Vol. 3 (4), pp. 240-253. Date of Electronic Publication: 2016 Aug 25.
Publication Year :
2016

Abstract

Recent cancer research has demonstrated the existence of circulating tumor cells (CTCs) in cancer patient's blood. Once identified, CTC biomarkers will be invaluable tools for clinical diagnosis, prognosis and treatment. In this review, we propose ex vivo culture as a rational strategy for large scale amplification of the limited numbers of CTCs from a patient sample, to derive enough CTCs for accurate and reproducible characterization of the biophysical, biochemical, gene expressional and behavioral properties of the harvested cells. Because of tumor cell heterogeneity, it is important to amplify all the CTCs in a blood sample for a comprehensive understanding of their role in cancer metastasis. By analyzing critical steps and technical issues in ex vivo CTC culture, we developed a cost-effective and reproducible protocol directly culturing whole peripheral blood mononuclear cells, relying on an assumed survival advantage in CTCs and CTC-like cells over the normal cells to amplify this specified cluster of cancer cells.

Details

Language :
English
ISSN :
2214-3882
Volume :
3
Issue :
4
Database :
MEDLINE
Journal :
Asian journal of urology
Publication Type :
Academic Journal
Accession number :
29264192
Full Text :
https://doi.org/10.1016/j.ajur.2016.08.005