A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system.

Authors :: Tang N
Zhang Y
Pedrera M
Chang P
Baigent S
Moffat K
Shen Z
Nair V
Yao Y
Source :: Vaccine [Vaccine] 2018 Jan 29; Vol. 36 (5), pp. 716-722. Date of Electronic Publication: 2018 Jan 02.
Publication Year :: 2018
Abstract: Herpesvirus of turkeys (HVT) has been successfully used as live vaccine against Marek's disease (MD) worldwide for more than 40 years either alone or in combination with other serotypes. HVT is also widely used as a vector platform for generation of recombinant vaccines against a number of avian diseases such as infectious bursal disease (IBD), Newcastle disease (ND) and avian influenza (AI) using conventional recombination methods or recombineering tools on cloned viral genomes. In the present study, we describe the application of CRISPR/Cas9-based genome editing as a rapid and efficient method of generating HVT recombinants expressing VP2 protein of IBDV. This approach offers an efficient method to introduce other viral antigens into the HVT genome for rapid development of recombinant vaccines.<br /> (Copyright © 2018 The Pirbright Institute. Published by Elsevier Ltd.. All rights reserved.)

Subjects :: Animals
Chick Embryo
Chickens
Fibroblasts
Gene Editing
Gene Expression
Gene Knock-In Techniques
Gene Order
Gene Targeting
Genes, Reporter
Genetic Engineering
Mice
Virus Replication
CRISPR-Cas Systems
Genetic Vectors genetics
Herpesvirus 1, Meleagrid genetics
Vaccines, Synthetic genetics
Vaccines, Synthetic immunology

Full Text Access

Tools