BRAF V600E accelerates disease progression and enhances immune suppression in a mouse model of B-cell leukemia.

Mutated mitogen-activated protein kinase (MAPK) pathway components promote tumor survival, proliferation, and immune evasion in solid tumors. MAPK mutations occur in hematologic cancers as well, but their role is less clear and few models are available to study this. We developed an in vivo model of disseminated BRAF ^V600E B-cell leukemia to determine the effects of this mutation on tumor development and immune evasion. Mice with B-cell-restricted BRAF ^V600E expression crossed with the Eµ-TCL1 model of chronic lymphocytic leukemia (CLL) developed leukemia significantly earlier (median, 4.9 vs 8.1 months; P < .001) and had significantly shorter lifespan (median, 7.3 vs 12.1 months; P < .001) versus BRAF wild-type counterparts. BRAF ^V600E expression did not affect B-cell proliferation but reduced spontaneous apoptosis. BRAF ^V600E -mutant leukemia produced greater T-cell effects, evidenced by exhaustion immunophenotype and CD44 ⁺ T-cell percentage, as well as increased expression of PD-L1 on CD11b ⁺ cells. Results were confirmed in syngeneic mice engrafted with BRAF ^V600E leukemia cells. Furthermore, a BRAF ^V600E -expressing CLL cell line more strongly inhibited anti-CD3/CD28-induced T-cell proliferation, which was reversed by BRAF ^V600E inhibition. These results demonstrate the immune-suppressive impact of BRAF ^V600E in B-cell leukemias and introduce a new model to develop rational combination strategies targeting both tumor cells and tumor-mediated immune evasion.
Competing Interests: Conflict-of-interest disclosure: The authors declare no competing financial interests.