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MiR-491-5p negatively regulates cell proliferation and motility by targeting PDGFRA in prostate cancer.
- Source :
-
American journal of cancer research [Am J Cancer Res] 2017 Dec 01; Vol. 7 (12), pp. 2545-2553. Date of Electronic Publication: 2017 Dec 01 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- MicroRNA-491-5p (miR-491-5p) has been implicated in several cancers; however, its role in human prostate cancer (PCa) remains unknown. In this study, we observed downregulation of miR-491-5p expression in PCa tissues and cell lines. CCK-8 and EdU assays showed that forced expression of miR-491-5p suppressed PCa cell proliferation, which was further confirmed in a cell cycle assay. Overexpression of miR-491-5p also reduced PCa cell migration and invasion abilities as indicated by Transwell assays. Additionally, miR-491-5p overexpression significantly inhibited PCa growth in a mouse xenograft model. Mechanistically, platelet-derived growth factor receptor α (PDGFRA) was found to be a novel target of miR-491-5p. Re-introduction of PDGFRA antagonized the inhibitory effects of miR-491-5p on the proliferation and motility abilities of PCa cells. In clinical samples of PCa, miR-491-5p was negatively correlated with PDGFRA expression, which was upregulated in PCa. Collectively, these results demonstrate that miR-491-5p acts as a tumor suppressor in PCa by directly targeting PDGFRA and may serve as a therapeutic biomarker for patients with PCa.<br />Competing Interests: None.
Details
- Language :
- English
- ISSN :
- 2156-6976
- Volume :
- 7
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- American journal of cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 29312807