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Contribution of Secondary Metabolites to the Gastroprotective Effect of Aqueous Extract of Ximenia americana L. (Olacaceae) Stem Bark in Rats.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2018 Jan 09; Vol. 23 (1). Date of Electronic Publication: 2018 Jan 09. - Publication Year :
- 2018
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Abstract
- Ximenia americana L. (Olacaceae) is used in ethnomedicine as cicatrizant and for the treatment of gastric disorders. This study identified the chemical constituents of the aqueous extract of X. americana (XaAE) and evaluated its antiulcerogenic activity. After lyophilization, XaAE was analyzed by liquid chromatography-mass spectrometry (LC-MS) and its antiulcerogenic effect was evaluated in acute gastric lesions induced by ethanol, acidified ethanol, and indomethacin. Antisecretory action, mucus production and the participation of sulfhydryl groups (-SH) and nitric oxide (NO) were also investigated. The chromatographic analysis identified procyanidins B and C and catechin/epicatechin as major compounds. Oral administration of XaAE (100, 200 and 400 mg/kg) inhibited the gastric lesions induced by ethanol (76.1%, 77.5% and 100%, respectively), acidified ethanol (44.9%, 80.6% and 94.9%, respectively) and indomethacin (56.4%, 52.7% and 64.9%, respectively). XaAE reduced gastric contents and acidity (51.4% and 67.7%, respectively) but did not alter the production of gastric mucus. The reduction of the -SH and NO groups promoted by N -ethylmaleimide (NEM) and N ω-nitro-l-arginine-methyl-ester (L-NAME) respectively, reduced the gastroprotective effect of XaAE. In conclusion, XaAE has gastroprotective activity mediated in part by -SH, NO and antisecretory activity. This antiulcer action was initially correlated to its major constituents, procyanidins B and C and catechin/epicatechin.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Animals
Arginine analogs & derivatives
Arginine chemistry
Catechin chemistry
Catechin pharmacology
Chromatography, High Pressure Liquid methods
Ethanol chemistry
Gastrointestinal Diseases chemically induced
Indomethacin chemistry
Mass Spectrometry methods
Mice
Phytotherapy methods
Plant Extracts isolation & purification
Proanthocyanidins chemistry
Proanthocyanidins pharmacology
Protective Agents chemistry
Protective Agents isolation & purification
Protective Agents pharmacology
Rats
Rats, Wistar
Stomach drug effects
Water
Gastrointestinal Diseases prevention & control
Olacaceae chemistry
Plant Extracts chemistry
Plant Extracts pharmacology
Secondary Metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 23
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 29315228
- Full Text :
- https://doi.org/10.3390/molecules23010112