Back to Search
Start Over
Protein corona and phospholipase activity drive selective accumulation of nanomicelles in atherosclerotic plaques.
- Source :
-
Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2018 Apr; Vol. 14 (3), pp. 643-650. Date of Electronic Publication: 2018 Jan 06. - Publication Year :
- 2018
-
Abstract
- ApoB-100 and Phosphatidylcholine-specific phospholipase C (PC-PLC) are important contributors to atherosclerosis development. ApoB-100 is the main structural protein of LDL, being directly associated with atherosclerosis plaque generation. PC-PLC is highly expressed in atherosclerosis lesions and contributes to their progression. We show how phosphatidylcholine-coated nanomicelles can be used for specific characterisation of atherosclerosis plaque. Results show that ApoB-100 in the protein corona of the nanomicelle targets the particles to atherosclerotic areas in apolipoprotein E <superscript>-/-</superscript> mice. Furthermore, PC-PLC selectively removes the polar heads from the phospholipid coating of the nanomicelles leading to their accumulation. To fully characterise the behaviour of the nanomicelles, we developed multimodal probes using a nanoemulsion step. Hybrid imaging revealed plaque accumulation of the nanomicelles and colocalisation with PC-PLC expression and ApoB-100 in the plaque. This study shows how protein corona composition and enzyme-driven nanomaterial accumulation can be used for detection of atherosclerosis.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Apolipoprotein B-100 metabolism
Mice
Mice, Knockout, ApoE
Nanocomposites administration & dosage
Plaque, Atherosclerotic pathology
Protein Corona chemistry
Type C Phospholipases chemistry
Apolipoproteins E physiology
Ferric Compounds chemistry
Micelles
Nanocomposites chemistry
Plaque, Atherosclerotic metabolism
Protein Corona metabolism
Type C Phospholipases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1549-9642
- Volume :
- 14
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Nanomedicine : nanotechnology, biology, and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 29317346
- Full Text :
- https://doi.org/10.1016/j.nano.2017.12.021