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Biogenesis of podosome rosettes through fission.
- Source :
-
Scientific reports [Sci Rep] 2018 Jan 11; Vol. 8 (1), pp. 524. Date of Electronic Publication: 2018 Jan 11. - Publication Year :
- 2018
-
Abstract
- Podosomes are dynamic actin-based membrane protrusions that are important for extracellular matrix degradation and invasive cell motility. Individual podosomes are often found to organize into large rosette-like structures in some types of cells, such as osteoclasts, endothelial cells, Src-transformed fibroblasts, and certain highly invasive cancer cells. In this study, we show that new podosome rosettes arise through one of two mechanisms; de novo assembly or fission of a pre-existing podosome rosette in Src-transformed fibroblasts. Fission is a more efficient way than de novo assembly to generate new podosome rosettes in these cells. Podosome rosettes undergoing fission possess higher motility and a stronger matrix-degrading capability. Podosome rosette fission may be the result of polarized myosin II-mediated contractility of these structures, which is coordinately regulated by myosin light chain kinase and Rho-associated kinase II. Collectively, this study unveils a previously unknown mechanism-fission for the biogenesis of podosome rosettes.
- Subjects :
- Actins metabolism
Amides pharmacology
Animals
Azepines pharmacology
Cell Line, Tumor
Cell Membrane metabolism
Cell Movement physiology
Humans
Mice
Microscopy, Fluorescence
Mutagenesis, Site-Directed
Myosin-Light-Chain Kinase antagonists & inhibitors
Myosin-Light-Chain Kinase genetics
Myosin-Light-Chain Kinase metabolism
NIH 3T3 Cells
Naphthalenes pharmacology
Podosomes drug effects
Pyridines pharmacology
RNA Interference
RNA, Small Interfering metabolism
Rosette Formation
rho-Associated Kinases antagonists & inhibitors
rho-Associated Kinases genetics
rho-Associated Kinases metabolism
src-Family Kinases genetics
src-Family Kinases metabolism
Podosomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 29323185
- Full Text :
- https://doi.org/10.1038/s41598-017-18861-2