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Carbamoyl-phosphate synthetases from Neurospora crassa. Immunological relatedness of the enzymes from Neurospora, bacteria, yeast, and mammals.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1985 Nov 15; Vol. 260 (26), pp. 14355-62. - Publication Year :
- 1985
-
Abstract
- Neurospora crassa contains two carbamoyl-phosphate synthetases: a mitochondrial enzyme (CPS-A) which supplies carbamoyl phosphate for arginine biosynthesis, and a nuclear enzyme whose product is used for the synthesis of pyrimidines. We have prepared antiserum against a highly purified preparation of the large subunit of CPS-A and have used the antiserum to demonstrate that the large subunit is, like most mitochondrially localized proteins, initially synthesized as a higher molecular weight precursor. The CPS-A antiserum cross-reacts with the nuclear enzyme, allowing us to identify the product of the complex N. crassa pyr-3 genetic locus as a protein with a subunit molecular weight of 180,000. Finally, we have found that the CPS-A antiserum also cross-reacts with carbamoyl-phosphate synthetases from bacteria, yeast, and mammals. The immunological relatedness of carbamoyl-phosphate synthetases from such diverse species suggests that the protein sequences required for carbamoyl phosphate production have been highly conserved during the course of evolution.
- Subjects :
- Animals
Antibody Specificity
Biological Evolution
Carbamoyl-Phosphate Synthase (Ammonia) analysis
Carbamoyl-Phosphate Synthase (Ammonia) isolation & purification
Cell Nucleus enzymology
Enzyme Precursors analysis
Escherichia coli enzymology
Immune Sera immunology
Immunosorbent Techniques
Kidney enzymology
Liver enzymology
Molecular Weight
Mutation
Rats
Saccharomyces cerevisiae enzymology
Carbamoyl-Phosphate Synthase (Ammonia) immunology
Ligases immunology
Mitochondria enzymology
Neurospora enzymology
Neurospora crassa enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 260
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 2932445