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Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles.
- Source :
-
Nature communications [Nat Commun] 2018 Jan 12; Vol. 9 (1), pp. 175. Date of Electronic Publication: 2018 Jan 12. - Publication Year :
- 2018
-
Abstract
- Extracellular vesicles (EVs) carry RNA, DNA, proteins, and lipids. Specifically, tumor-derived EVs have the potential to be utilized as disease-specific biomarkers. However, a lack of methods to isolate tumor-specific EVs has limited their use in clinical settings. Here we report a sensitive analytical microfluidic platform ( <superscript>EV</superscript> HB-Chip) that enables tumor-specific EV-RNA isolation within 3 h. Using the <superscript>EV</superscript> HB-Chip, we achieve 94% tumor-EV specificity, a limit of detection of 100 EVs per μL, and a 10-fold increase in tumor RNA enrichment in comparison to other methods. Our approach allows for the subsequent release of captured tumor EVs, enabling downstream characterization and functional studies. Processing serum and plasma samples from glioblastoma multiforme (GBM) patients, we can detect the mutant EGFRvIII mRNA. Moreover, using next-generation RNA sequencing, we identify genes specific to GBM as well as transcripts that are hallmarks for the four genetic subtypes of the disease.
- Subjects :
- Biological Transport
Brain Neoplasms chemistry
Brain Neoplasms genetics
Cell Line, Tumor
ErbB Receptors genetics
ErbB Receptors metabolism
Extracellular Vesicles metabolism
Glioblastoma chemistry
Glioblastoma genetics
Humans
Microfluidics instrumentation
RNA genetics
RNA metabolism
Brain Neoplasms metabolism
Extracellular Vesicles chemistry
Glioblastoma metabolism
Microfluidics methods
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 29330365
- Full Text :
- https://doi.org/10.1038/s41467-017-02261-1