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Development of a novel equine influenza virus live-attenuated vaccine.
- Source :
-
Virology [Virology] 2018 Mar; Vol. 516, pp. 76-85. Date of Electronic Publication: 2018 Jan 11. - Publication Year :
- 2018
-
Abstract
- H3N8 equine influenza virus (EIV) is an important and significant respiratory pathogen of horses. EIV is enzootic in Europe and North America, mainly due to the suboptimal efficacy of current vaccines. We describe, for the first time, the generation of a temperature sensitive (ts) H3N8 EIV live-attenuated influenza vaccine (LAIV) using reverse-genetics approaches. Our EIV LAIV was attenuated (att) in vivo and able to induce, upon a single intranasal administration, protection against H3N8 EIV wild-type (WT) challenge in both a mouse model and the natural host, the horse. Notably, since our EIV LAIV was generated using reverse genetics, the vaccine can be easily updated against drifting or emerging strains of EIV using the safety backbone of our EIV LAIV as master donor virus (MDV). These results demonstrate the feasibility of implementing a novel EIV LAIV approach for the prevention and control of currently circulating H3N8 EIVs in horse populations.<br /> (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antibodies, Viral immunology
Female
Horse Diseases immunology
Horse Diseases virology
Horses
Influenza A Virus, H3N8 Subtype genetics
Influenza Vaccines administration & dosage
Influenza Vaccines genetics
Male
Mice
Mice, Inbred C57BL
Orthomyxoviridae Infections prevention & control
Orthomyxoviridae Infections virology
Reverse Genetics
Vaccination
Vaccines, Attenuated administration & dosage
Vaccines, Attenuated genetics
Horse Diseases prevention & control
Influenza A Virus, H3N8 Subtype immunology
Influenza Vaccines immunology
Orthomyxoviridae Infections veterinary
Vaccines, Attenuated immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 516
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 29331866
- Full Text :
- https://doi.org/10.1016/j.virol.2018.01.005